Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25485

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25475 and BPHYT_RS25480 are separated by 173 nucleotidesBPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotidesBPHYT_RS25485 and BPHYT_RS25490 overlap by 11 nucleotidesBPHYT_RS25490 and BPHYT_RS25495 are separated by 32 nucleotides BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064 _RS25475 BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879 _RS25480 BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750 _RS25485 BPHYT_RS25490: BPHYT_RS25490 - hypothetical protein, at 1,295,740 to 1,296,663 _RS25490 BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007 _RS25495 Position (kb) 1294 1295 1296Strain fitness (log2 ratio) -2 -1 0 1at 1294.151 kb on + strandat 1294.353 kb on + strand, within BPHYT_RS25480at 1294.355 kb on + strand, within BPHYT_RS25480at 1294.508 kb on - strand, within BPHYT_RS25480at 1294.511 kb on + strand, within BPHYT_RS25480at 1294.512 kb on - strand, within BPHYT_RS25480at 1295.848 kb on - strand, within BPHYT_RS25490at 1295.982 kb on + strand, within BPHYT_RS25490at 1296.035 kb on + strand, within BPHYT_RS25490at 1296.084 kb on + strand, within BPHYT_RS25490at 1296.495 kb on + strand, within BPHYT_RS25490at 1296.498 kb on - strand, within BPHYT_RS25490at 1296.654 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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1,294,151 + -0.1
1,294,353 + BPHYT_RS25480 0.18 -0.7
1,294,355 + BPHYT_RS25480 0.18 -1.9
1,294,508 - BPHYT_RS25480 0.42 +0.2
1,294,511 + BPHYT_RS25480 0.43 -0.6
1,294,512 - BPHYT_RS25480 0.43 +1.2
1,295,848 - BPHYT_RS25490 0.12 +0.9
1,295,982 + BPHYT_RS25490 0.26 +0.2
1,296,035 + BPHYT_RS25490 0.32 +0.2
1,296,084 + BPHYT_RS25490 0.37 +0.6
1,296,495 + BPHYT_RS25490 0.82 -1.5
1,296,498 - BPHYT_RS25490 0.82 -1.1
1,296,654 + -0.8

Or see this region's nucleotide sequence