Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23020 and BPHYT_RS23025 overlap by 4 nucleotides BPHYT_RS23025 and BPHYT_RS23030 overlap by 1 nucleotides
BPHYT_RS23020: BPHYT_RS23020 - alcohol dehydrogenase, at 724,604 to 725,959
_RS23020
BPHYT_RS23025: BPHYT_RS23025 - GMC family oxidoreductase, at 725,956 to 727,734
_RS23025
BPHYT_RS23030: BPHYT_RS23030 - gluconate 2-dehydrogenase, at 727,734 to 728,474
_RS23030
Position (kb) 725
726
727
728 Strain fitness (log2 ratio) -2
-1
0
1
2 at 725.547 kb on + strand, within BPHYT_RS23020 at 725.583 kb on + strand, within BPHYT_RS23020 at 725.584 kb on - strand, within BPHYT_RS23020 at 725.959 kb on + strand at 726.053 kb on + strand at 726.095 kb on - strand at 726.228 kb on + strand, within BPHYT_RS23025 at 726.229 kb on - strand, within BPHYT_RS23025 at 726.230 kb on + strand, within BPHYT_RS23025 at 726.291 kb on - strand, within BPHYT_RS23025 at 726.990 kb on - strand, within BPHYT_RS23025 at 726.990 kb on - strand, within BPHYT_RS23025 at 727.072 kb on - strand, within BPHYT_RS23025 at 727.143 kb on - strand, within BPHYT_RS23025 at 727.214 kb on - strand, within BPHYT_RS23025 at 727.556 kb on + strand, within BPHYT_RS23025 at 727.556 kb on + strand, within BPHYT_RS23025 at 727.557 kb on - strand at 727.557 kb on - strand at 727.557 kb on - strand at 727.557 kb on - strand at 728.143 kb on + strand, within BPHYT_RS23030 at 728.436 kb on + strand at 728.456 kb on + strand at 728.457 kb on - strand at 728.482 kb on + strand at 728.483 kb on - strand at 728.483 kb on - strand at 728.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 725,547 + BPHYT_RS23020 0.70 -0.5 725,583 + BPHYT_RS23020 0.72 +0.1 725,584 - BPHYT_RS23020 0.72 -1.1 725,959 + +0.1 726,053 + -0.7 726,095 - +0.1 726,228 + BPHYT_RS23025 0.15 -0.8 726,229 - BPHYT_RS23025 0.15 -1.2 726,230 + BPHYT_RS23025 0.15 +0.2 726,291 - BPHYT_RS23025 0.19 +0.8 726,990 - BPHYT_RS23025 0.58 -1.1 726,990 - BPHYT_RS23025 0.58 +0.3 727,072 - BPHYT_RS23025 0.63 -0.4 727,143 - BPHYT_RS23025 0.67 -0.8 727,214 - BPHYT_RS23025 0.71 -0.9 727,556 + BPHYT_RS23025 0.90 -1.2 727,556 + BPHYT_RS23025 0.90 -2.1 727,557 - -1.7 727,557 - -1.0 727,557 - -0.8 727,557 - +0.3 728,143 + BPHYT_RS23030 0.55 -0.4 728,436 + +0.0 728,456 + -1.4 728,457 - +0.3 728,482 + -0.8 728,483 - -0.3 728,483 - +1.9 728,585 - -0.7
Or see this region's nucleotide sequence