Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22530 and BPHYT_RS22535 are separated by 18 nucleotides BPHYT_RS22535 and BPHYT_RS22540 overlap by 8 nucleotides BPHYT_RS22540 and BPHYT_RS22545 are separated by 155 nucleotides
BPHYT_RS22530: BPHYT_RS22530 - N5,N10-methylene tetrahydromethanopterin reductase, at 615,943 to 617,349
_RS22530
BPHYT_RS22535: BPHYT_RS22535 - methionine ABC transporter ATP-binding protein, at 617,368 to 618,528
_RS22535
BPHYT_RS22540: BPHYT_RS22540 - metal ABC transporter permease, at 618,521 to 619,177
_RS22540
BPHYT_RS22545: BPHYT_RS22545 - acyl-CoA dehydrogenase, at 619,333 to 620,535
_RS22545
Position (kb) 617
618
619 Strain fitness (log2 ratio) -1
0
1 at 616.913 kb on - strand, within BPHYT_RS22530 at 617.347 kb on + strand at 617.961 kb on + strand, within BPHYT_RS22535 at 618.787 kb on - strand, within BPHYT_RS22540 at 618.976 kb on + strand, within BPHYT_RS22540 at 619.181 kb on + strand at 619.181 kb on + strand at 619.250 kb on - strand at 619.250 kb on - strand at 619.257 kb on + strand at 619.257 kb on + strand at 619.257 kb on + strand at 619.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 616,913 - BPHYT_RS22530 0.69 -0.8 617,347 + -0.0 617,961 + BPHYT_RS22535 0.51 +0.4 618,787 - BPHYT_RS22540 0.40 +1.6 618,976 + BPHYT_RS22540 0.69 +0.3 619,181 + +1.2 619,181 + +0.4 619,250 - -0.6 619,250 - -0.4 619,257 + +0.3 619,257 + -0.7 619,257 + +0.6 619,261 + +1.0
Or see this region's nucleotide sequence