Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21620

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21610 and BPHYT_RS21615 are separated by 117 nucleotidesBPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotidesBPHYT_RS21620 and BPHYT_RS21625 are separated by 117 nucleotides BPHYT_RS21610: BPHYT_RS21610 - DNA-binding protein, at 404,332 to 405,861 _RS21610 BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653 _RS21615 BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117 _RS21620 BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149 _RS21625 Position (kb) 406 407 408Strain fitness (log2 ratio) -1 0 1at 405.872 kb on - strandat 405.872 kb on - strandat 405.997 kb on - strandat 405.997 kb on - strandat 406.184 kb on + strand, within BPHYT_RS21615at 406.735 kb on + strand, within BPHYT_RS21620at 407.320 kb on + strandat 407.321 kb on - strandat 407.623 kb on + strand, within BPHYT_RS21625at 408.109 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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405,872 - -1.7
405,872 - -1.2
405,997 - -1.0
405,997 - -1.5
406,184 + BPHYT_RS21615 0.30 -0.8
406,735 + BPHYT_RS21620 0.18 -1.5
407,320 + -0.3
407,321 - -0.8
407,623 + BPHYT_RS21625 0.42 +0.6
408,109 - +0.1

Or see this region's nucleotide sequence