Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21430 and BPHYT_RS21435 overlap by 4 nucleotides BPHYT_RS21435 and BPHYT_RS21440 are separated by 517 nucleotides BPHYT_RS21440 and BPHYT_RS21445 are separated by 18 nucleotides
BPHYT_RS21430: BPHYT_RS21430 - 3-oxoadipate enol-lactonase, at 366,462 to 367,253
_RS21430
BPHYT_RS21435: BPHYT_RS21435 - 4-carboxymuconolactone decarboxylase, at 367,250 to 367,645
_RS21435
BPHYT_RS21440: BPHYT_RS21440 - sugar ABC transporter permease, at 368,163 to 369,011
_RS21440
BPHYT_RS21445: BPHYT_RS21445 - sugar ABC transporter ATP-binding protein, at 369,030 to 370,382
_RS21445
Position (kb) 368
369
370 Strain fitness (log2 ratio) -2
-1
0
1 at 367.644 kb on - strand at 367.750 kb on + strand at 368.171 kb on + strand at 368.514 kb on + strand, within BPHYT_RS21440 at 368.514 kb on + strand, within BPHYT_RS21440 at 368.515 kb on - strand, within BPHYT_RS21440 at 368.627 kb on - strand, within BPHYT_RS21440 at 368.749 kb on - strand, within BPHYT_RS21440 at 368.920 kb on + strand, within BPHYT_RS21440 at 369.096 kb on + strand at 369.097 kb on - strand at 369.097 kb on - strand at 369.097 kb on - strand at 369.125 kb on + strand at 369.501 kb on + strand, within BPHYT_RS21445 at 369.501 kb on + strand, within BPHYT_RS21445 at 369.502 kb on - strand, within BPHYT_RS21445
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 367,644 - +0.4 367,750 + -1.6 368,171 + -0.5 368,514 + BPHYT_RS21440 0.41 -0.9 368,514 + BPHYT_RS21440 0.41 -2.1 368,515 - BPHYT_RS21440 0.41 -0.6 368,627 - BPHYT_RS21440 0.55 -0.1 368,749 - BPHYT_RS21440 0.69 -1.2 368,920 + BPHYT_RS21440 0.89 -0.6 369,096 + -0.3 369,097 - -1.1 369,097 - +1.4 369,097 - +0.3 369,125 + +0.0 369,501 + BPHYT_RS21445 0.35 -1.4 369,501 + BPHYT_RS21445 0.35 -0.6 369,502 - BPHYT_RS21445 0.35 +0.0
Or see this region's nucleotide sequence