Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21060

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21055 and BPHYT_RS21060 are separated by 202 nucleotides BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733 _RS21055 BPHYT_RS21060: BPHYT_RS21060 - hypothetical protein, at 284,936 to 286,408 _RS21060 Position (kb) 284 285 286 287Strain fitness (log2 ratio) -2 -1 0 1 2 3at 284.007 kb on + strand, within BPHYT_RS21055at 284.131 kb on - strand, within BPHYT_RS21055at 284.264 kb on - strand, within BPHYT_RS21055at 284.497 kb on + strand, within BPHYT_RS21055at 284.668 kb on - strandat 284.668 kb on - strandat 284.675 kb on - strandat 284.810 kb on - strandat 285.011 kb on - strandat 285.257 kb on + strand, within BPHYT_RS21060at 285.262 kb on - strand, within BPHYT_RS21060at 285.294 kb on + strand, within BPHYT_RS21060at 285.295 kb on - strand, within BPHYT_RS21060at 285.971 kb on + strand, within BPHYT_RS21060at 286.214 kb on - strand, within BPHYT_RS21060at 286.454 kb on + strandat 286.480 kb on - strandat 286.506 kb on - strandat 286.567 kb on - strandat 286.567 kb on - strandat 286.577 kb on - strandat 286.662 kb on + strandat 287.013 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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284,007 + BPHYT_RS21055 0.13 -1.5
284,131 - BPHYT_RS21055 0.27 +3.1
284,264 - BPHYT_RS21055 0.43 -0.4
284,497 + BPHYT_RS21055 0.71 +0.4
284,668 - -0.6
284,668 - -0.3
284,675 - -0.1
284,810 - +0.0
285,011 - -0.6
285,257 + BPHYT_RS21060 0.22 +0.1
285,262 - BPHYT_RS21060 0.22 +0.0
285,294 + BPHYT_RS21060 0.24 -0.0
285,295 - BPHYT_RS21060 0.24 -0.1
285,971 + BPHYT_RS21060 0.70 -0.1
286,214 - BPHYT_RS21060 0.87 -0.5
286,454 + +0.3
286,480 - -2.2
286,506 - -1.0
286,567 - -0.7
286,567 - -0.3
286,577 - -1.6
286,662 + -0.2
287,013 - -0.0

Or see this region's nucleotide sequence