Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20205 and BPHYT_RS20210 overlap by 1 nucleotides BPHYT_RS20210 and BPHYT_RS20215 are separated by 474 nucleotides
BPHYT_RS20205: BPHYT_RS20205 - alcohol dehydrogenase, at 78,863 to 80,023
_RS20205
BPHYT_RS20210: BPHYT_RS20210 - antibiotic biosynthesis monooxygenase, at 80,023 to 80,331
_RS20210
BPHYT_RS20215: BPHYT_RS20215 - RNA polymerase sigma 70, at 80,806 to 81,477
_RS20215
Position (kb)
78
79
80
81 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 77.959 kb on + strand at 77.959 kb on + strand at 77.978 kb on - strand at 77.996 kb on - strand at 78.077 kb on - strand at 78.167 kb on - strand at 78.185 kb on - strand at 78.194 kb on - strand at 78.338 kb on - strand at 78.460 kb on + strand at 78.503 kb on - strand at 78.522 kb on + strand at 78.534 kb on - strand at 78.539 kb on - strand at 78.676 kb on + strand at 78.677 kb on - strand at 78.766 kb on - strand at 78.787 kb on + strand at 78.844 kb on + strand at 78.878 kb on - strand at 78.919 kb on + strand at 79.098 kb on - strand, within BPHYT_RS20205 at 79.098 kb on - strand, within BPHYT_RS20205 at 79.411 kb on - strand, within BPHYT_RS20205 at 79.411 kb on - strand, within BPHYT_RS20205 at 79.411 kb on - strand, within BPHYT_RS20205 at 79.411 kb on - strand, within BPHYT_RS20205 at 79.653 kb on + strand, within BPHYT_RS20205 at 79.654 kb on - strand, within BPHYT_RS20205 at 79.654 kb on - strand, within BPHYT_RS20205 at 80.046 kb on + strand at 80.167 kb on + strand, within BPHYT_RS20210 at 80.318 kb on + strand at 80.319 kb on - strand at 80.319 kb on - strand at 80.355 kb on + strand at 80.426 kb on + strand at 80.506 kb on + strand at 80.507 kb on - strand at 80.685 kb on - strand at 80.722 kb on + strand at 80.723 kb on - strand at 80.806 kb on - strand at 80.806 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 77,959 + -1.9 77,959 + +0.9 77,978 - +0.2 77,996 - -1.4 78,077 - +0.2 78,167 - -0.6 78,185 - -0.2 78,194 - +0.7 78,338 - +0.8 78,460 + -2.8 78,503 - -0.8 78,522 + +0.3 78,534 - -0.2 78,539 - -0.0 78,676 + +1.1 78,677 - -0.5 78,766 - -1.1 78,787 + -0.8 78,844 + +1.1 78,878 - -1.5 78,919 + -0.7 79,098 - BPHYT_RS20205 0.20 -0.3 79,098 - BPHYT_RS20205 0.20 -1.7 79,411 - BPHYT_RS20205 0.47 -0.7 79,411 - BPHYT_RS20205 0.47 -0.6 79,411 - BPHYT_RS20205 0.47 -0.8 79,411 - BPHYT_RS20205 0.47 +0.2 79,653 + BPHYT_RS20205 0.68 -1.6 79,654 - BPHYT_RS20205 0.68 -0.3 79,654 - BPHYT_RS20205 0.68 -1.5 80,046 + +0.2 80,167 + BPHYT_RS20210 0.47 -0.3 80,318 + -0.5 80,319 - +0.5 80,319 - +3.2 80,355 + -0.7 80,426 + -1.4 80,506 + -0.5 80,507 - -0.1 80,685 - +1.0 80,722 + +0.2 80,723 - -0.3 80,806 - -0.5 80,806 - -0.6
Or see this region's nucleotide sequence