Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20205

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20205 and BPHYT_RS20210 overlap by 1 nucleotidesBPHYT_RS20210 and BPHYT_RS20215 are separated by 474 nucleotides BPHYT_RS20205: BPHYT_RS20205 - alcohol dehydrogenase, at 78,863 to 80,023 _RS20205 BPHYT_RS20210: BPHYT_RS20210 - antibiotic biosynthesis monooxygenase, at 80,023 to 80,331 _RS20210 BPHYT_RS20215: BPHYT_RS20215 - RNA polymerase sigma 70, at 80,806 to 81,477 _RS20215 Position (kb) 78 79 80 81Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 77.959 kb on + strandat 77.959 kb on + strandat 77.978 kb on - strandat 77.996 kb on - strandat 78.077 kb on - strandat 78.167 kb on - strandat 78.185 kb on - strandat 78.194 kb on - strandat 78.338 kb on - strandat 78.460 kb on + strandat 78.503 kb on - strandat 78.522 kb on + strandat 78.534 kb on - strandat 78.539 kb on - strandat 78.676 kb on + strandat 78.677 kb on - strandat 78.766 kb on - strandat 78.787 kb on + strandat 78.844 kb on + strandat 78.878 kb on - strandat 78.919 kb on + strandat 79.098 kb on - strand, within BPHYT_RS20205at 79.098 kb on - strand, within BPHYT_RS20205at 79.411 kb on - strand, within BPHYT_RS20205at 79.411 kb on - strand, within BPHYT_RS20205at 79.411 kb on - strand, within BPHYT_RS20205at 79.411 kb on - strand, within BPHYT_RS20205at 79.653 kb on + strand, within BPHYT_RS20205at 79.654 kb on - strand, within BPHYT_RS20205at 79.654 kb on - strand, within BPHYT_RS20205at 80.046 kb on + strandat 80.167 kb on + strand, within BPHYT_RS20210at 80.318 kb on + strandat 80.319 kb on - strandat 80.319 kb on - strandat 80.355 kb on + strandat 80.426 kb on + strandat 80.506 kb on + strandat 80.507 kb on - strandat 80.685 kb on - strandat 80.722 kb on + strandat 80.723 kb on - strandat 80.806 kb on - strandat 80.806 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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77,959 + -1.9
77,959 + +0.9
77,978 - +0.2
77,996 - -1.4
78,077 - +0.2
78,167 - -0.6
78,185 - -0.2
78,194 - +0.7
78,338 - +0.8
78,460 + -2.8
78,503 - -0.8
78,522 + +0.3
78,534 - -0.2
78,539 - -0.0
78,676 + +1.1
78,677 - -0.5
78,766 - -1.1
78,787 + -0.8
78,844 + +1.1
78,878 - -1.5
78,919 + -0.7
79,098 - BPHYT_RS20205 0.20 -0.3
79,098 - BPHYT_RS20205 0.20 -1.7
79,411 - BPHYT_RS20205 0.47 -0.7
79,411 - BPHYT_RS20205 0.47 -0.6
79,411 - BPHYT_RS20205 0.47 -0.8
79,411 - BPHYT_RS20205 0.47 +0.2
79,653 + BPHYT_RS20205 0.68 -1.6
79,654 - BPHYT_RS20205 0.68 -0.3
79,654 - BPHYT_RS20205 0.68 -1.5
80,046 + +0.2
80,167 + BPHYT_RS20210 0.47 -0.3
80,318 + -0.5
80,319 - +0.5
80,319 - +3.2
80,355 + -0.7
80,426 + -1.4
80,506 + -0.5
80,507 - -0.1
80,685 - +1.0
80,722 + +0.2
80,723 - -0.3
80,806 - -0.5
80,806 - -0.6

Or see this region's nucleotide sequence