Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20050

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20045 and BPHYT_RS20050 are separated by 117 nucleotidesBPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotidesBPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotides BPHYT_RS20045: BPHYT_RS20045 - farnesyl-diphosphate farnesyltransferase, at 42,130 to 43,176 _RS20045 BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542 _RS20050 BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202 _RS20055 BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122 _RS20060 Position (kb) 43 44Strain fitness (log2 ratio) -2 -1 0 1 2at 43.219 kb on + strandat 43.242 kb on - strandat 43.312 kb on - strandat 43.776 kb on + strandat 43.843 kb on - strandat 43.886 kb on + strandat 44.069 kb on + strand, within BPHYT_RS20055at 44.069 kb on + strand, within BPHYT_RS20055at 44.236 kb on + strandat 44.360 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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43,219 + +1.2
43,242 - +2.5
43,312 - -0.0
43,776 + +0.9
43,843 - +0.4
43,886 + +0.5
44,069 + BPHYT_RS20055 0.58 +2.1
44,069 + BPHYT_RS20055 0.58 -2.0
44,236 + -0.4
44,360 - +0.7

Or see this region's nucleotide sequence