Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19975 and BPHYT_RS19980 are separated by 29 nucleotides BPHYT_RS19980 and BPHYT_RS19985 are separated by 2 nucleotides BPHYT_RS19985 and BPHYT_RS19990 are separated by 41 nucleotides
BPHYT_RS19975: BPHYT_RS19975 - IclR family transcriptional regulator, at 24,912 to 25,676
_RS19975
BPHYT_RS19980: BPHYT_RS19980 - citrate synthase, at 25,706 to 26,530
_RS19980
BPHYT_RS19985: BPHYT_RS19985 - CoA-transferase, at 26,533 to 27,732
_RS19985
BPHYT_RS19990: BPHYT_RS19990 - MFS transporter, at 27,774 to 29,207
_RS19990
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 25.674 kb on + strand at 25.737 kb on + strand at 26.172 kb on + strand, within BPHYT_RS19980 at 26.172 kb on + strand, within BPHYT_RS19980 at 26.173 kb on - strand, within BPHYT_RS19980 at 27.308 kb on + strand, within BPHYT_RS19985 at 27.663 kb on - strand at 27.694 kb on + strand at 27.695 kb on - strand at 28.661 kb on - strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 25,674 + +0.5 25,737 + +1.0 26,172 + BPHYT_RS19980 0.56 -1.3 26,172 + BPHYT_RS19980 0.56 +0.1 26,173 - BPHYT_RS19980 0.57 -0.1 27,308 + BPHYT_RS19985 0.65 -0.1 27,663 - -0.1 27,694 + -0.0 27,695 - -0.1 28,661 - BPHYT_RS19990 0.62 -0.4 28,726 + BPHYT_RS19990 0.66 +0.3 28,726 + BPHYT_RS19990 0.66 -2.5 28,726 + BPHYT_RS19990 0.66 -0.2 28,727 - BPHYT_RS19990 0.66 -0.8 28,727 - BPHYT_RS19990 0.66 -0.7
Or see this region's nucleotide sequence