Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14130 and BPHYT_RS14135 are separated by 17 nucleotides BPHYT_RS14135 and BPHYT_RS14140 overlap by 4 nucleotides BPHYT_RS14140 and BPHYT_RS14145 are separated by 148 nucleotides
BPHYT_RS14130: BPHYT_RS14130 - phosphate ABC transporter ATP-binding protein, at 3,226,702 to 3,227,550
_RS14130
BPHYT_RS14135: BPHYT_RS14135 - phosphate transporter permease subunit PtsA, at 3,227,568 to 3,228,464
_RS14135
BPHYT_RS14140: BPHYT_RS14140 - phosphate transporter permease subunit PstC, at 3,228,461 to 3,229,462
_RS14140
BPHYT_RS14145: BPHYT_RS14145 - phosphate ABC transporter substrate-binding protein, at 3,229,611 to 3,230,642
_RS14145
Position (kb)
3228
3229
3230 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3227.544 kb on + strand at 3227.569 kb on + strand at 3227.570 kb on - strand at 3227.570 kb on - strand at 3227.689 kb on + strand, within BPHYT_RS14135 at 3227.689 kb on + strand, within BPHYT_RS14135 at 3228.300 kb on - strand, within BPHYT_RS14135 at 3228.444 kb on - strand at 3228.619 kb on + strand, within BPHYT_RS14140 at 3228.619 kb on + strand, within BPHYT_RS14140 at 3228.620 kb on - strand, within BPHYT_RS14140 at 3228.997 kb on - strand, within BPHYT_RS14140 at 3229.194 kb on + strand, within BPHYT_RS14140 at 3229.195 kb on - strand, within BPHYT_RS14140 at 3229.195 kb on - strand, within BPHYT_RS14140 at 3229.531 kb on - strand at 3229.572 kb on - strand at 3229.572 kb on - strand at 3229.575 kb on + strand at 3229.575 kb on + strand at 3229.575 kb on + strand at 3229.576 kb on - strand at 3229.576 kb on - strand at 3229.613 kb on - strand at 3229.613 kb on - strand at 3229.702 kb on + strand at 3229.703 kb on - strand at 3229.703 kb on - strand at 3229.703 kb on - strand at 3229.703 kb on - strand at 3229.703 kb on - strand at 3229.868 kb on - strand, within BPHYT_RS14145 at 3229.964 kb on - strand, within BPHYT_RS14145 at 3229.964 kb on - strand at 3229.964 kb on - strand, within BPHYT_RS14145 at 3230.138 kb on - strand, within BPHYT_RS14145 at 3230.269 kb on + strand, within BPHYT_RS14145 at 3230.269 kb on + strand, within BPHYT_RS14145 at 3230.269 kb on + strand at 3230.269 kb on + strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145 at 3230.270 kb on - strand, within BPHYT_RS14145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 3,227,544 + +1.7 3,227,569 + +0.1 3,227,570 - +1.5 3,227,570 - +1.2 3,227,689 + BPHYT_RS14135 0.13 +0.7 3,227,689 + BPHYT_RS14135 0.13 +0.9 3,228,300 - BPHYT_RS14135 0.82 +1.5 3,228,444 - +1.8 3,228,619 + BPHYT_RS14140 0.16 -0.0 3,228,619 + BPHYT_RS14140 0.16 +1.0 3,228,620 - BPHYT_RS14140 0.16 +4.1 3,228,997 - BPHYT_RS14140 0.53 +3.8 3,229,194 + BPHYT_RS14140 0.73 +0.8 3,229,195 - BPHYT_RS14140 0.73 +3.9 3,229,195 - BPHYT_RS14140 0.73 +4.7 3,229,531 - +0.1 3,229,572 - +0.2 3,229,572 - +0.2 3,229,575 + +0.1 3,229,575 + +0.2 3,229,575 + +1.9 3,229,576 - +0.9 3,229,576 - +1.5 3,229,613 - +1.4 3,229,613 - +1.5 3,229,702 + +0.8 3,229,703 - +2.3 3,229,703 - +2.7 3,229,703 - +1.1 3,229,703 - +1.6 3,229,703 - +1.6 3,229,868 - BPHYT_RS14145 0.25 +1.9 3,229,964 - BPHYT_RS14145 0.34 +3.2 3,229,964 - +3.3 3,229,964 - BPHYT_RS14145 0.34 +1.2 3,230,138 - BPHYT_RS14145 0.51 +2.8 3,230,269 + BPHYT_RS14145 0.64 -2.4 3,230,269 + BPHYT_RS14145 0.64 +0.4 3,230,269 + -0.0 3,230,269 + BPHYT_RS14145 0.64 -1.7 3,230,270 - BPHYT_RS14145 0.64 +1.6 3,230,270 - BPHYT_RS14145 0.64 +2.5 3,230,270 - BPHYT_RS14145 0.64 +1.4 3,230,270 - BPHYT_RS14145 0.64 +1.9 3,230,270 - BPHYT_RS14145 0.64 +1.7 3,230,270 - BPHYT_RS14145 0.64 +2.1
Or see this region's nucleotide sequence