Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12265

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12260 and BPHYT_RS12265 overlap by 14 nucleotidesBPHYT_RS12265 and BPHYT_RS12270 overlap by 4 nucleotides BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401 _RS12260 BPHYT_RS12265: BPHYT_RS12265 - alanine acetyltransferase, at 2,801,388 to 2,801,882 _RS12265 BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709 _RS12270 Position (kb) 2801 2802Strain fitness (log2 ratio) -2 -1 0 1 2at 2801.516 kb on - strand, within BPHYT_RS12265at 2801.744 kb on - strand, within BPHYT_RS12265at 2801.853 kb on - strandat 2801.853 kb on - strandat 2801.855 kb on - strandat 2802.721 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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2,801,516 - BPHYT_RS12265 0.26 +2.3
2,801,744 - BPHYT_RS12265 0.72 -1.5
2,801,853 - -0.5
2,801,853 - +0.2
2,801,855 - -2.0
2,802,721 + +0.2

Or see this region's nucleotide sequence