Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09980

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09970 and BPHYT_RS09975 are separated by 139 nucleotidesBPHYT_RS09975 and BPHYT_RS09980 are separated by 125 nucleotidesBPHYT_RS09980 and BPHYT_RS09985 are separated by 44 nucleotides BPHYT_RS09970: BPHYT_RS09970 - glutathione S-transferase, at 2,270,388 to 2,271,011 _RS09970 BPHYT_RS09975: BPHYT_RS09975 - hypothetical protein, at 2,271,151 to 2,271,495 _RS09975 BPHYT_RS09980: BPHYT_RS09980 - TetR family transcriptional regulator, at 2,271,621 to 2,272,262 _RS09980 BPHYT_RS09985: BPHYT_RS09985 - trans-2-enoyl-CoA reductase, at 2,272,307 to 2,273,503 _RS09985 Position (kb) 2271 2272 2273Strain fitness (log2 ratio) -1 0 1at 2270.716 kb on - strand, within BPHYT_RS09970at 2270.802 kb on - strand, within BPHYT_RS09970at 2270.949 kb on + strandat 2270.950 kb on - strandat 2271.603 kb on - strandat 2271.983 kb on + strand, within BPHYT_RS09980at 2272.258 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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2,270,716 - BPHYT_RS09970 0.53 -1.5
2,270,802 - BPHYT_RS09970 0.66 -0.2
2,270,949 + -0.8
2,270,950 - -1.3
2,271,603 - +0.4
2,271,983 + BPHYT_RS09980 0.56 -1.3
2,272,258 + -1.2

Or see this region's nucleotide sequence