Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09805 and BPHYT_RS09810 are separated by 5 nucleotides BPHYT_RS09810 and BPHYT_RS09815 are separated by 153 nucleotides BPHYT_RS09815 and BPHYT_RS09820 are separated by 121 nucleotides BPHYT_RS09820 and BPHYT_RS09825 are separated by 400 nucleotides
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
BPHYT_RS09810: BPHYT_RS09810 - hypothetical protein, at 2,228,597 to 2,228,821
_RS09810
BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149
_RS09815
BPHYT_RS09820: BPHYT_RS09820 - hypothetical protein, at 2,231,271 to 2,231,675
_RS09820
BPHYT_RS09825: BPHYT_RS09825 - Fe-S protein, at 2,232,076 to 2,232,951
_RS09825
Position (kb)
2228
2229
2230
2231
2232 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2228.082 kb on + strand, within BPHYT_RS09805 at 2228.091 kb on + strand, within BPHYT_RS09805 at 2228.092 kb on - strand, within BPHYT_RS09805 at 2228.157 kb on + strand, within BPHYT_RS09805 at 2228.232 kb on + strand, within BPHYT_RS09805 at 2228.236 kb on - strand, within BPHYT_RS09805 at 2228.237 kb on + strand, within BPHYT_RS09805 at 2228.238 kb on - strand, within BPHYT_RS09805 at 2228.495 kb on - strand, within BPHYT_RS09805 at 2228.548 kb on - strand at 2228.548 kb on - strand at 2228.691 kb on - strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.802 kb on - strand at 2229.984 kb on + strand, within BPHYT_RS09815 at 2230.571 kb on - strand, within BPHYT_RS09815 at 2230.879 kb on + strand, within BPHYT_RS09815 at 2230.881 kb on + strand, within BPHYT_RS09815 at 2230.881 kb on + strand, within BPHYT_RS09815 at 2231.678 kb on + strand at 2231.701 kb on + strand at 2232.095 kb on + strand at 2232.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 2,228,082 + BPHYT_RS09805 0.36 +1.8 2,228,091 + BPHYT_RS09805 0.37 -0.5 2,228,092 - BPHYT_RS09805 0.37 +2.6 2,228,157 + BPHYT_RS09805 0.45 +0.6 2,228,232 + BPHYT_RS09805 0.55 +2.4 2,228,236 - BPHYT_RS09805 0.55 -0.2 2,228,237 + BPHYT_RS09805 0.56 -0.2 2,228,238 - BPHYT_RS09805 0.56 -2.3 2,228,495 - BPHYT_RS09805 0.88 +0.3 2,228,548 - -0.6 2,228,548 - +0.2 2,228,691 - BPHYT_RS09810 0.42 +1.4 2,228,762 + BPHYT_RS09810 0.73 +0.0 2,228,762 + BPHYT_RS09810 0.73 +0.3 2,228,802 - -0.5 2,229,984 + BPHYT_RS09815 0.46 -1.6 2,230,571 - BPHYT_RS09815 0.73 +1.4 2,230,879 + BPHYT_RS09815 0.88 +0.4 2,230,881 + BPHYT_RS09815 0.88 +0.4 2,230,881 + BPHYT_RS09815 0.88 +4.3 2,231,678 + +0.0 2,231,701 + -0.9 2,232,095 + +0.9 2,232,096 - -0.7
Or see this region's nucleotide sequence