Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02540 and BPHYT_RS02545 are separated by 415 nucleotides BPHYT_RS02545 and BPHYT_RS02550 are separated by 44 nucleotides BPHYT_RS02550 and BPHYT_RS02555 are separated by 236 nucleotides
BPHYT_RS02540: BPHYT_RS02540 - hypothetical protein, at 577,383 to 577,637
_RS02540
BPHYT_RS02545: BPHYT_RS02545 - nitrogen regulatory protein P-II 1, at 578,053 to 578,391
_RS02545
BPHYT_RS02550: BPHYT_RS02550 - ammonia channel protein, at 578,436 to 579,944
_RS02550
BPHYT_RS02555: BPHYT_RS02555 - glutamate--cysteine ligase, at 580,181 to 581,470
_RS02555
Position (kb) 578
579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 577.461 kb on - strand, within BPHYT_RS02540 at 577.614 kb on - strand at 577.725 kb on - strand at 577.791 kb on + strand at 577.791 kb on + strand at 577.792 kb on - strand at 577.792 kb on - strand at 577.792 kb on - strand at 577.792 kb on - strand at 577.805 kb on - strand at 577.805 kb on - strand at 578.018 kb on - strand at 578.038 kb on + strand at 578.039 kb on - strand at 578.189 kb on - strand, within BPHYT_RS02545 at 578.204 kb on - strand, within BPHYT_RS02545 at 578.204 kb on - strand, within BPHYT_RS02545 at 578.390 kb on - strand at 578.403 kb on - strand at 578.414 kb on + strand at 578.415 kb on - strand at 578.415 kb on - strand at 578.446 kb on + strand at 578.811 kb on + strand, within BPHYT_RS02550 at 578.812 kb on - strand, within BPHYT_RS02550 at 578.907 kb on + strand, within BPHYT_RS02550 at 578.907 kb on + strand, within BPHYT_RS02550 at 578.908 kb on - strand at 578.979 kb on + strand, within BPHYT_RS02550 at 578.979 kb on + strand, within BPHYT_RS02550 at 578.979 kb on + strand, within BPHYT_RS02550 at 578.979 kb on + strand, within BPHYT_RS02550 at 578.980 kb on - strand, within BPHYT_RS02550 at 579.049 kb on - strand, within BPHYT_RS02550 at 579.058 kb on - strand, within BPHYT_RS02550 at 579.117 kb on - strand, within BPHYT_RS02550 at 579.117 kb on - strand, within BPHYT_RS02550 at 579.117 kb on - strand, within BPHYT_RS02550 at 579.139 kb on + strand, within BPHYT_RS02550 at 579.353 kb on + strand, within BPHYT_RS02550 at 579.353 kb on + strand, within BPHYT_RS02550 at 579.354 kb on - strand, within BPHYT_RS02550 at 579.822 kb on + strand at 579.840 kb on + strand at 579.841 kb on - strand at 579.841 kb on - strand at 579.942 kb on + strand at 579.943 kb on - strand at 579.943 kb on - strand at 579.985 kb on - strand at 580.041 kb on + strand at 580.041 kb on + strand at 580.048 kb on + strand at 580.048 kb on + strand at 580.048 kb on + strand at 580.049 kb on - strand at 580.069 kb on + strand at 580.070 kb on - strand at 580.070 kb on - strand at 580.081 kb on - strand at 580.149 kb on - strand at 580.194 kb on + strand at 580.195 kb on - strand at 580.195 kb on - strand at 580.308 kb on - strand at 580.505 kb on + strand, within BPHYT_RS02555 at 580.757 kb on + strand, within BPHYT_RS02555 at 580.757 kb on + strand, within BPHYT_RS02555 at 580.758 kb on - strand at 580.758 kb on - strand, within BPHYT_RS02555 at 580.792 kb on - strand, within BPHYT_RS02555 at 580.820 kb on + strand, within BPHYT_RS02555 at 580.883 kb on + strand, within BPHYT_RS02555 at 580.884 kb on - strand, within BPHYT_RS02555 at 580.884 kb on - strand, within BPHYT_RS02555
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 577,461 - BPHYT_RS02540 0.31 +1.2 577,614 - +1.6 577,725 - +1.2 577,791 + +1.2 577,791 + -0.6 577,792 - -0.2 577,792 - -1.5 577,792 - +0.2 577,792 - -0.4 577,805 - -0.1 577,805 - +0.4 578,018 - +0.1 578,038 + -0.2 578,039 - -1.2 578,189 - BPHYT_RS02545 0.40 +0.5 578,204 - BPHYT_RS02545 0.45 +0.4 578,204 - BPHYT_RS02545 0.45 -3.5 578,390 - -1.5 578,403 - -1.2 578,414 + -0.8 578,415 - +1.0 578,415 - -0.2 578,446 + -0.5 578,811 + BPHYT_RS02550 0.25 -0.6 578,812 - BPHYT_RS02550 0.25 -1.4 578,907 + BPHYT_RS02550 0.31 -0.8 578,907 + BPHYT_RS02550 0.31 -1.3 578,908 - +1.4 578,979 + BPHYT_RS02550 0.36 -2.8 578,979 + BPHYT_RS02550 0.36 -0.2 578,979 + BPHYT_RS02550 0.36 -1.2 578,979 + BPHYT_RS02550 0.36 -0.1 578,980 - BPHYT_RS02550 0.36 -0.3 579,049 - BPHYT_RS02550 0.41 -2.0 579,058 - BPHYT_RS02550 0.41 +0.1 579,117 - BPHYT_RS02550 0.45 -0.8 579,117 - BPHYT_RS02550 0.45 +0.1 579,117 - BPHYT_RS02550 0.45 +0.2 579,139 + BPHYT_RS02550 0.47 -0.8 579,353 + BPHYT_RS02550 0.61 +0.0 579,353 + BPHYT_RS02550 0.61 -1.0 579,354 - BPHYT_RS02550 0.61 -0.2 579,822 + -0.1 579,840 + +0.0 579,841 - +0.3 579,841 - -0.3 579,942 + +0.9 579,943 - -0.5 579,943 - +0.7 579,985 - -1.2 580,041 + -0.0 580,041 + +0.1 580,048 + -0.6 580,048 + -0.1 580,048 + -0.2 580,049 - -0.1 580,069 + +0.2 580,070 - -1.0 580,070 - -2.4 580,081 - -0.1 580,149 - +0.1 580,194 + -1.0 580,195 - -0.6 580,195 - +1.2 580,308 - -0.7 580,505 + BPHYT_RS02555 0.25 -1.2 580,757 + BPHYT_RS02555 0.45 +0.4 580,757 + BPHYT_RS02555 0.45 -1.4 580,758 - -1.1 580,758 - BPHYT_RS02555 0.45 +0.4 580,792 - BPHYT_RS02555 0.47 +0.8 580,820 + BPHYT_RS02555 0.50 +0.0 580,883 + BPHYT_RS02555 0.54 +0.7 580,884 - BPHYT_RS02555 0.54 -0.3 580,884 - BPHYT_RS02555 0.54 +0.1
Or see this region's nucleotide sequence