Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02130 and BPHYT_RS02135 are separated by 60 nucleotides BPHYT_RS02135 and BPHYT_RS02140 are separated by 347 nucleotides
BPHYT_RS02130: BPHYT_RS02130 - hydroxymethylglutaryl-CoA lyase, at 488,141 to 489,067
_RS02130
BPHYT_RS02135: BPHYT_RS02135 - glyoxylate reductase, at 489,128 to 490,069
_RS02135
BPHYT_RS02140: BPHYT_RS02140 - alpha/beta hydrolase, at 490,417 to 491,019
_RS02140
Position (kb) 489
490
491 Strain fitness (log2 ratio) -2
-1
0
1
2 at 488.299 kb on - strand, within BPHYT_RS02130 at 488.299 kb on - strand, within BPHYT_RS02130 at 489.079 kb on + strand at 489.080 kb on - strand at 489.124 kb on - strand at 489.405 kb on + strand, within BPHYT_RS02135 at 489.405 kb on + strand, within BPHYT_RS02135 at 489.763 kb on - strand, within BPHYT_RS02135 at 489.818 kb on - strand, within BPHYT_RS02135 at 490.183 kb on + strand at 490.184 kb on - strand at 490.184 kb on - strand at 490.189 kb on + strand at 490.574 kb on + strand, within BPHYT_RS02140 at 490.575 kb on - strand, within BPHYT_RS02140 at 490.638 kb on + strand, within BPHYT_RS02140 at 490.638 kb on + strand, within BPHYT_RS02140 at 490.639 kb on - strand, within BPHYT_RS02140 at 490.639 kb on - strand, within BPHYT_RS02140 at 490.663 kb on + strand, within BPHYT_RS02140 at 490.664 kb on - strand, within BPHYT_RS02140 at 490.685 kb on - strand, within BPHYT_RS02140 at 490.890 kb on + strand, within BPHYT_RS02140 at 490.891 kb on - strand, within BPHYT_RS02140 at 491.000 kb on + strand at 491.000 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 488,299 - BPHYT_RS02130 0.17 -1.1 488,299 - BPHYT_RS02130 0.17 +0.8 489,079 + +0.1 489,080 - -2.5 489,124 - -0.7 489,405 + BPHYT_RS02135 0.29 -0.4 489,405 + BPHYT_RS02135 0.29 +0.0 489,763 - BPHYT_RS02135 0.67 -1.0 489,818 - BPHYT_RS02135 0.73 -1.7 490,183 + -0.4 490,184 - +0.0 490,184 - -0.8 490,189 + -0.8 490,574 + BPHYT_RS02140 0.26 +0.1 490,575 - BPHYT_RS02140 0.26 -1.4 490,638 + BPHYT_RS02140 0.37 +1.9 490,638 + BPHYT_RS02140 0.37 +0.1 490,639 - BPHYT_RS02140 0.37 -0.1 490,639 - BPHYT_RS02140 0.37 -1.3 490,663 + BPHYT_RS02140 0.41 -0.1 490,664 - BPHYT_RS02140 0.41 +0.1 490,685 - BPHYT_RS02140 0.44 -0.4 490,890 + BPHYT_RS02140 0.78 +0.1 490,891 - BPHYT_RS02140 0.79 -2.4 491,000 + +0.3 491,000 + -0.7
Or see this region's nucleotide sequence