Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02025 and BPHYT_RS02030 are separated by 122 nucleotides BPHYT_RS02030 and BPHYT_RS02035 are separated by 331 nucleotides BPHYT_RS02035 and BPHYT_RS02040 are separated by 80 nucleotides
BPHYT_RS02025: BPHYT_RS02025 - peptide ABC transporter permease, at 463,326 to 465,038
_RS02025
BPHYT_RS02030: BPHYT_RS02030 - potassium channel protein, at 465,161 to 466,120
_RS02030
BPHYT_RS02035: BPHYT_RS02035 - hypothetical protein, at 466,452 to 466,697
_RS02035
BPHYT_RS02040: BPHYT_RS02040 - hypothetical protein, at 466,778 to 467,008
_RS02040
Position (kb) 465
466
467 Strain fitness (log2 ratio) -2
-1
0
1
2 at 464.347 kb on + strand, within BPHYT_RS02025 at 465.286 kb on - strand, within BPHYT_RS02030 at 465.433 kb on + strand, within BPHYT_RS02030 at 465.434 kb on - strand, within BPHYT_RS02030 at 465.536 kb on - strand, within BPHYT_RS02030 at 465.759 kb on + strand, within BPHYT_RS02030 at 465.760 kb on - strand, within BPHYT_RS02030 at 465.880 kb on - strand, within BPHYT_RS02030 at 465.880 kb on - strand, within BPHYT_RS02030 at 465.969 kb on + strand, within BPHYT_RS02030 at 465.969 kb on + strand, within BPHYT_RS02030 at 465.970 kb on - strand, within BPHYT_RS02030 at 466.306 kb on - strand at 466.472 kb on + strand at 466.473 kb on - strand at 466.666 kb on + strand, within BPHYT_RS02035 at 466.667 kb on - strand, within BPHYT_RS02035 at 466.870 kb on - strand, within BPHYT_RS02040 at 466.977 kb on + strand, within BPHYT_RS02040 at 466.978 kb on - strand, within BPHYT_RS02040 at 466.978 kb on - strand, within BPHYT_RS02040 at 466.992 kb on + strand at 466.993 kb on - strand at 467.049 kb on + strand at 467.050 kb on - strand at 467.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 464,347 + BPHYT_RS02025 0.60 -0.6 465,286 - BPHYT_RS02030 0.13 +0.6 465,433 + BPHYT_RS02030 0.28 -0.0 465,434 - BPHYT_RS02030 0.28 -0.0 465,536 - BPHYT_RS02030 0.39 -0.2 465,759 + BPHYT_RS02030 0.62 +1.1 465,760 - BPHYT_RS02030 0.62 +0.5 465,880 - BPHYT_RS02030 0.75 -1.3 465,880 - BPHYT_RS02030 0.75 -0.2 465,969 + BPHYT_RS02030 0.84 -0.3 465,969 + BPHYT_RS02030 0.84 -0.4 465,970 - BPHYT_RS02030 0.84 -1.5 466,306 - -1.6 466,472 + -0.0 466,473 - -1.1 466,666 + BPHYT_RS02035 0.87 -0.3 466,667 - BPHYT_RS02035 0.87 +1.2 466,870 - BPHYT_RS02040 0.40 -0.4 466,977 + BPHYT_RS02040 0.86 -1.0 466,978 - BPHYT_RS02040 0.87 +1.0 466,978 - BPHYT_RS02040 0.87 -0.3 466,992 + -0.8 466,993 - -1.8 467,049 + +0.2 467,050 - -0.3 467,050 - +2.2
Or see this region's nucleotide sequence