Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS01290

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS01285 and BPHYT_RS01290 are separated by 107 nucleotidesBPHYT_RS01290 and BPHYT_RS01295 are separated by 61 nucleotidesBPHYT_RS01295 and BPHYT_RS01300 are separated by 87 nucleotides BPHYT_RS01285: BPHYT_RS01285 - polyketide cyclase, at 294,403 to 294,813 _RS01285 BPHYT_RS01290: BPHYT_RS01290 - FAD dependent oxidoreductase, at 294,921 to 296,360 _RS01290 BPHYT_RS01295: BPHYT_RS01295 - glutathione S-transferase, at 296,422 to 297,096 _RS01295 BPHYT_RS01300: BPHYT_RS01300 - ArsR family transcriptional regulator, at 297,184 to 297,879 _RS01300 Position (kb) 294 295 296 297Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 294.040 kb on + strandat 294.067 kb on - strandat 294.166 kb on + strandat 294.167 kb on - strandat 294.183 kb on - strandat 294.210 kb on - strandat 294.301 kb on + strandat 294.302 kb on - strandat 294.306 kb on - strandat 294.337 kb on - strandat 294.383 kb on + strandat 294.383 kb on + strandat 294.384 kb on - strandat 294.384 kb on - strandat 294.384 kb on - strandat 294.384 kb on - strandat 294.384 kb on - strandat 294.384 kb on - strandat 294.439 kb on + strandat 294.439 kb on + strandat 294.494 kb on + strand, within BPHYT_RS01285at 294.494 kb on + strand, within BPHYT_RS01285at 294.496 kb on + strand, within BPHYT_RS01285at 294.497 kb on - strand, within BPHYT_RS01285at 294.497 kb on - strand, within BPHYT_RS01285at 294.497 kb on - strandat 294.541 kb on + strand, within BPHYT_RS01285at 294.542 kb on - strand, within BPHYT_RS01285at 294.640 kb on + strandat 294.776 kb on - strandat 294.841 kb on + strandat 294.880 kb on - strandat 294.892 kb on - strandat 294.892 kb on - strandat 295.094 kb on + strand, within BPHYT_RS01290at 295.094 kb on + strand, within BPHYT_RS01290at 295.095 kb on - strand, within BPHYT_RS01290at 295.097 kb on + strand, within BPHYT_RS01290at 295.097 kb on + strand, within BPHYT_RS01290at 295.098 kb on - strand, within BPHYT_RS01290at 295.152 kb on + strand, within BPHYT_RS01290at 295.153 kb on - strand, within BPHYT_RS01290at 295.218 kb on + strand, within BPHYT_RS01290at 295.284 kb on - strand, within BPHYT_RS01290at 295.285 kb on + strand, within BPHYT_RS01290at 295.285 kb on + strand, within BPHYT_RS01290at 295.286 kb on - strand, within BPHYT_RS01290at 295.286 kb on - strand, within BPHYT_RS01290at 295.336 kb on + strand, within BPHYT_RS01290at 295.353 kb on - strand, within BPHYT_RS01290at 295.353 kb on - strand, within BPHYT_RS01290at 295.486 kb on - strand, within BPHYT_RS01290at 295.486 kb on - strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.857 kb on + strand, within BPHYT_RS01290at 295.858 kb on - strand, within BPHYT_RS01290at 295.858 kb on - strand, within BPHYT_RS01290at 295.858 kb on - strand, within BPHYT_RS01290at 295.858 kb on - strand, within BPHYT_RS01290at 295.858 kb on - strand, within BPHYT_RS01290at 295.859 kb on + strand, within BPHYT_RS01290at 295.860 kb on - strand, within BPHYT_RS01290at 295.992 kb on + strand, within BPHYT_RS01290at 295.992 kb on + strand, within BPHYT_RS01290at 296.149 kb on - strand, within BPHYT_RS01290at 296.278 kb on - strandat 296.292 kb on - strandat 296.307 kb on + strandat 296.308 kb on - strandat 296.335 kb on + strandat 296.437 kb on + strandat 296.460 kb on + strandat 296.460 kb on + strandat 296.461 kb on - strandat 296.480 kb on + strandat 296.639 kb on - strand, within BPHYT_RS01295at 296.747 kb on + strand, within BPHYT_RS01295at 296.747 kb on + strand, within BPHYT_RS01295at 296.748 kb on - strand, within BPHYT_RS01295at 296.819 kb on + strand, within BPHYT_RS01295at 296.826 kb on + strand, within BPHYT_RS01295at 297.059 kb on + strandat 297.094 kb on + strandat 297.193 kb on - strandat 297.196 kb on - strandat 297.339 kb on - strand, within BPHYT_RS01300

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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294,040 + +0.2
294,067 - -2.2
294,166 + -0.1
294,167 - +0.4
294,183 - +1.0
294,210 - -0.2
294,301 + +0.3
294,302 - +0.6
294,306 - -0.3
294,337 - -1.7
294,383 + +1.4
294,383 + -0.8
294,384 - +1.1
294,384 - +0.1
294,384 - +0.1
294,384 - +0.6
294,384 - -0.4
294,384 - -1.5
294,439 + +2.3
294,439 + -1.0
294,494 + BPHYT_RS01285 0.22 -0.5
294,494 + BPHYT_RS01285 0.22 +0.6
294,496 + BPHYT_RS01285 0.23 -0.6
294,497 - BPHYT_RS01285 0.23 -0.7
294,497 - BPHYT_RS01285 0.23 +1.4
294,497 - -0.9
294,541 + BPHYT_RS01285 0.34 -0.1
294,542 - BPHYT_RS01285 0.34 -0.4
294,640 + +0.4
294,776 - -0.8
294,841 + -0.5
294,880 - -1.5
294,892 - -1.7
294,892 - -1.5
295,094 + BPHYT_RS01290 0.12 -0.0
295,094 + BPHYT_RS01290 0.12 -0.8
295,095 - BPHYT_RS01290 0.12 -1.1
295,097 + BPHYT_RS01290 0.12 +0.7
295,097 + BPHYT_RS01290 0.12 -1.0
295,098 - BPHYT_RS01290 0.12 -0.4
295,152 + BPHYT_RS01290 0.16 -2.4
295,153 - BPHYT_RS01290 0.16 +0.2
295,218 + BPHYT_RS01290 0.21 +1.1
295,284 - BPHYT_RS01290 0.25 -1.2
295,285 + BPHYT_RS01290 0.25 -0.6
295,285 + BPHYT_RS01290 0.25 -0.0
295,286 - BPHYT_RS01290 0.25 +0.0
295,286 - BPHYT_RS01290 0.25 +0.2
295,336 + BPHYT_RS01290 0.29 -1.2
295,353 - BPHYT_RS01290 0.30 -2.2
295,353 - BPHYT_RS01290 0.30 +0.7
295,486 - BPHYT_RS01290 0.39 +0.2
295,486 - BPHYT_RS01290 0.39 -0.2
295,857 + BPHYT_RS01290 0.65 +0.0
295,857 + BPHYT_RS01290 0.65 +0.3
295,857 + BPHYT_RS01290 0.65 +1.0
295,857 + BPHYT_RS01290 0.65 +2.1
295,857 + BPHYT_RS01290 0.65 +0.4
295,857 + BPHYT_RS01290 0.65 -0.6
295,858 - BPHYT_RS01290 0.65 -3.3
295,858 - BPHYT_RS01290 0.65 +0.5
295,858 - BPHYT_RS01290 0.65 +0.8
295,858 - BPHYT_RS01290 0.65 +1.0
295,858 - BPHYT_RS01290 0.65 +0.2
295,859 + BPHYT_RS01290 0.65 -0.9
295,860 - BPHYT_RS01290 0.65 -0.1
295,992 + BPHYT_RS01290 0.74 -1.0
295,992 + BPHYT_RS01290 0.74 -0.7
296,149 - BPHYT_RS01290 0.85 -0.7
296,278 - +0.3
296,292 - +0.3
296,307 + +0.2
296,308 - +0.4
296,335 + -0.4
296,437 + -0.7
296,460 + -0.4
296,460 + -0.3
296,461 - -1.6
296,480 + +0.4
296,639 - BPHYT_RS01295 0.32 -1.9
296,747 + BPHYT_RS01295 0.48 +0.3
296,747 + BPHYT_RS01295 0.48 +1.0
296,748 - BPHYT_RS01295 0.48 +0.3
296,819 + BPHYT_RS01295 0.59 +1.4
296,826 + BPHYT_RS01295 0.60 -0.5
297,059 + -0.9
297,094 + +3.9
297,193 - +0.8
297,196 - +0.3
297,339 - BPHYT_RS01300 0.22 -2.0

Or see this region's nucleotide sequence