Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00715 and BPHYT_RS00720 are separated by 54 nucleotides BPHYT_RS00720 and BPHYT_RS00725 overlap by 4 nucleotides BPHYT_RS00725 and BPHYT_RS00730 are separated by 81 nucleotides
BPHYT_RS00715: BPHYT_RS00715 - branched-chain amino acid ABC transporter substrate-binding protein, at 166,337 to 167,515
_RS00715
BPHYT_RS00720: BPHYT_RS00720 - amidase, at 167,570 to 168,997
_RS00720
BPHYT_RS00725: BPHYT_RS00725 - hypothetical protein, at 168,994 to 169,539
_RS00725
BPHYT_RS00730: BPHYT_RS00730 - porin, at 169,621 to 170,655
_RS00730
Position (kb) 167
168
169 Strain fitness (log2 ratio) -1
0
1 at 166.648 kb on + strand, within BPHYT_RS00715 at 167.157 kb on - strand, within BPHYT_RS00715 at 167.205 kb on - strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.216 kb on + strand, within BPHYT_RS00715 at 167.283 kb on - strand, within BPHYT_RS00715 at 167.517 kb on + strand at 167.517 kb on + strand at 167.517 kb on + strand at 167.659 kb on - strand at 168.492 kb on - strand, within BPHYT_RS00720 at 168.495 kb on - strand, within BPHYT_RS00720 at 168.695 kb on - strand, within BPHYT_RS00720 at 168.851 kb on - strand, within BPHYT_RS00720 at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 169.287 kb on - strand, within BPHYT_RS00725 at 169.482 kb on - strand, within BPHYT_RS00725 at 169.616 kb on + strand at 169.617 kb on - strand at 169.624 kb on + strand at 169.624 kb on + strand at 169.718 kb on + strand at 169.912 kb on + strand, within BPHYT_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 166,648 + BPHYT_RS00715 0.26 +0.2 167,157 - BPHYT_RS00715 0.70 +0.5 167,205 - BPHYT_RS00715 0.74 +0.4 167,206 + BPHYT_RS00715 0.74 -0.2 167,206 + BPHYT_RS00715 0.74 +0.1 167,216 + BPHYT_RS00715 0.75 -0.7 167,283 - BPHYT_RS00715 0.80 -1.5 167,517 + +1.6 167,517 + +0.1 167,517 + +1.0 167,659 - -0.5 168,492 - BPHYT_RS00720 0.65 -1.0 168,495 - BPHYT_RS00720 0.65 -0.6 168,695 - BPHYT_RS00720 0.79 -1.4 168,851 - BPHYT_RS00720 0.90 +0.9 168,921 - -0.3 168,921 - +0.5 168,921 - +0.8 168,921 - +0.6 168,921 - -1.5 168,921 - -0.6 169,287 - BPHYT_RS00725 0.54 -1.5 169,482 - BPHYT_RS00725 0.89 -1.2 169,616 + -0.9 169,617 - -0.4 169,624 + -1.2 169,624 + -0.6 169,718 + -1.1 169,912 + BPHYT_RS00730 0.28 -1.6
Or see this region's nucleotide sequence