Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00210 and BPHYT_RS00215 are separated by 10 nucleotides BPHYT_RS00215 and BPHYT_RS00220 are separated by 5 nucleotides BPHYT_RS00220 and BPHYT_RS00225 are separated by 169 nucleotides BPHYT_RS00225 and BPHYT_RS00230 overlap by 4 nucleotides
BPHYT_RS00210: BPHYT_RS00210 - allantoate amidohydrolase, at 55,474 to 56,766
_RS00210
BPHYT_RS00215: BPHYT_RS00215 - aminoglycoside phosphotransferase, at 56,777 to 57,727
_RS00215
BPHYT_RS00220: BPHYT_RS00220 - choline kinase, at 57,733 to 58,674
_RS00220
BPHYT_RS00225: BPHYT_RS00225 - hypothetical protein, at 58,844 to 59,089
_RS00225
BPHYT_RS00230: BPHYT_RS00230 - sodium:solute symporter, at 59,086 to 60,612
_RS00230
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1 at 56.849 kb on - strand at 56.958 kb on - strand, within BPHYT_RS00215 at 57.044 kb on + strand, within BPHYT_RS00215 at 57.045 kb on - strand, within BPHYT_RS00215 at 57.543 kb on - strand, within BPHYT_RS00215 at 57.601 kb on - strand, within BPHYT_RS00215 at 57.601 kb on - strand, within BPHYT_RS00215 at 57.660 kb on + strand at 57.725 kb on + strand at 57.725 kb on + strand at 57.901 kb on + strand, within BPHYT_RS00220 at 58.014 kb on + strand, within BPHYT_RS00220 at 58.314 kb on + strand, within BPHYT_RS00220 at 58.315 kb on - strand, within BPHYT_RS00220 at 58.400 kb on - strand, within BPHYT_RS00220 at 58.469 kb on - strand, within BPHYT_RS00220 at 58.597 kb on + strand at 58.612 kb on + strand at 58.671 kb on - strand at 58.770 kb on + strand at 58.931 kb on + strand, within BPHYT_RS00225 at 59.037 kb on - strand, within BPHYT_RS00225 at 59.164 kb on + strand at 59.324 kb on - strand, within BPHYT_RS00230 at 59.362 kb on + strand, within BPHYT_RS00230 at 59.363 kb on - strand, within BPHYT_RS00230 at 59.363 kb on - strand, within BPHYT_RS00230 at 59.658 kb on + strand, within BPHYT_RS00230 at 59.659 kb on - strand, within BPHYT_RS00230 at 59.659 kb on - strand, within BPHYT_RS00230
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 56,849 - +0.6 56,958 - BPHYT_RS00215 0.19 +0.6 57,044 + BPHYT_RS00215 0.28 +1.7 57,045 - BPHYT_RS00215 0.28 +0.8 57,543 - BPHYT_RS00215 0.81 -1.1 57,601 - BPHYT_RS00215 0.87 +1.4 57,601 - BPHYT_RS00215 0.87 +0.2 57,660 + -0.1 57,725 + -1.0 57,725 + -0.0 57,901 + BPHYT_RS00220 0.18 -0.0 58,014 + BPHYT_RS00220 0.30 -0.8 58,314 + BPHYT_RS00220 0.62 +0.6 58,315 - BPHYT_RS00220 0.62 -0.3 58,400 - BPHYT_RS00220 0.71 -0.4 58,469 - BPHYT_RS00220 0.78 -0.1 58,597 + -1.2 58,612 + +0.7 58,671 - -2.3 58,770 + -1.4 58,931 + BPHYT_RS00225 0.35 -0.5 59,037 - BPHYT_RS00225 0.78 -0.7 59,164 + +1.7 59,324 - BPHYT_RS00230 0.16 +0.3 59,362 + BPHYT_RS00230 0.18 -0.2 59,363 - BPHYT_RS00230 0.18 -0.3 59,363 - BPHYT_RS00230 0.18 -1.0 59,658 + BPHYT_RS00230 0.37 -0.3 59,659 - BPHYT_RS00230 0.38 +0.6 59,659 - BPHYT_RS00230 0.38 -0.4
Or see this region's nucleotide sequence