Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34225 and BPHYT_RS34230 are separated by 105 nucleotides BPHYT_RS34230 and BPHYT_RS34235 are separated by 66 nucleotides BPHYT_RS34235 and BPHYT_RS34240 are separated by 25 nucleotides
BPHYT_RS34225: BPHYT_RS34225 - L-fucose dehydrogenase (EC 1.1.1.122) (from data) , at 3,280,406 to 3,281,446
_RS34225
BPHYT_RS34230: BPHYT_RS34230 - L-fuconate dehydratase; L-rhamnonate dehydratase (EC 4.2.1.68; EC 4.2.1.90) (from data) , at 3,281,552 to 3,282,847
_RS34230
BPHYT_RS34235: BPHYT_RS34235 - putative accessory domain for L-fuconate/L-rhamnonate dehydratase (EC 4.2.1.68; EC 4.2.1.90) (from data) , at 3,282,914 to 3,283,246
_RS34235
BPHYT_RS34240: BPHYT_RS34240 - ABC transporter for L-rhamnose/L-fucose/xylitol, permease component (from data) , at 3,283,272 to 3,284,327
_RS34240
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-2
-1
0
1 at 3280.609 kb on + strand, within BPHYT_RS34225 at 3280.668 kb on + strand, within BPHYT_RS34225 at 3280.668 kb on + strand, within BPHYT_RS34225 at 3280.668 kb on + strand, within BPHYT_RS34225 at 3280.730 kb on + strand, within BPHYT_RS34225 at 3280.801 kb on + strand, within BPHYT_RS34225 at 3280.801 kb on + strand, within BPHYT_RS34225 at 3280.801 kb on + strand, within BPHYT_RS34225 at 3281.085 kb on + strand, within BPHYT_RS34225 at 3281.092 kb on - strand, within BPHYT_RS34225 at 3281.474 kb on + strand at 3281.475 kb on - strand at 3281.637 kb on + strand at 3281.637 kb on + strand at 3281.637 kb on + strand at 3281.637 kb on + strand at 3281.803 kb on - strand, within BPHYT_RS34230 at 3282.141 kb on + strand, within BPHYT_RS34230 at 3282.525 kb on + strand, within BPHYT_RS34230 at 3282.526 kb on - strand, within BPHYT_RS34230 at 3282.526 kb on - strand, within BPHYT_RS34230 at 3282.759 kb on + strand at 3282.810 kb on + strand at 3282.986 kb on - strand, within BPHYT_RS34235 at 3283.232 kb on + strand at 3283.531 kb on + strand, within BPHYT_RS34240 at 3283.531 kb on + strand at 3283.655 kb on - strand, within BPHYT_RS34240
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 3,280,609 + BPHYT_RS34225 0.20 +0.4 3,280,668 + BPHYT_RS34225 0.25 +0.2 3,280,668 + BPHYT_RS34225 0.25 +0.1 3,280,668 + BPHYT_RS34225 0.25 +0.9 3,280,730 + BPHYT_RS34225 0.31 -0.6 3,280,801 + BPHYT_RS34225 0.38 +0.1 3,280,801 + BPHYT_RS34225 0.38 -0.2 3,280,801 + BPHYT_RS34225 0.38 -1.9 3,281,085 + BPHYT_RS34225 0.65 +0.5 3,281,092 - BPHYT_RS34225 0.66 +1.1 3,281,474 + -1.7 3,281,475 - -0.6 3,281,637 + -0.1 3,281,637 + -1.0 3,281,637 + -1.6 3,281,637 + -0.5 3,281,803 - BPHYT_RS34230 0.19 +0.9 3,282,141 + BPHYT_RS34230 0.45 +0.7 3,282,525 + BPHYT_RS34230 0.75 +0.1 3,282,526 - BPHYT_RS34230 0.75 -0.1 3,282,526 - BPHYT_RS34230 0.75 +1.4 3,282,759 + -0.1 3,282,810 + +0.2 3,282,986 - BPHYT_RS34235 0.22 -1.1 3,283,232 + +0.2 3,283,531 + BPHYT_RS34240 0.25 -0.5 3,283,531 + +0.9 3,283,655 - BPHYT_RS34240 0.36 -0.2
Or see this region's nucleotide sequence