Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34055

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34045 and BPHYT_RS34050 are separated by 3 nucleotidesBPHYT_RS34050 and BPHYT_RS34055 overlap by 4 nucleotidesBPHYT_RS34055 and BPHYT_RS34060 overlap by 4 nucleotidesBPHYT_RS34060 and BPHYT_RS34065 overlap by 1 nucleotides BPHYT_RS34045: BPHYT_RS34045 - secretion system protein F, at 3,238,817 to 3,239,665 _RS34045 BPHYT_RS34050: BPHYT_RS34050 - secretion system protein F, at 3,239,669 to 3,240,550 _RS34050 BPHYT_RS34055: BPHYT_RS34055 - hypothetical protein, at 3,240,547 to 3,240,897 _RS34055 BPHYT_RS34060: BPHYT_RS34060 - pilus biosynthesis protein TadE, at 3,240,894 to 3,241,730 _RS34060 BPHYT_RS34065: BPHYT_RS34065 - lytic transglycosylase, at 3,241,730 to 3,242,545 _RS34065 Position (kb) 3240 3241Strain fitness (log2 ratio) -1 0 1at 3239.912 kb on - strand, within BPHYT_RS34050at 3240.056 kb on + strand, within BPHYT_RS34050at 3240.125 kb on + strand, within BPHYT_RS34050at 3240.126 kb on - strand, within BPHYT_RS34050at 3240.305 kb on - strand, within BPHYT_RS34050at 3240.566 kb on - strandat 3241.444 kb on - strand, within BPHYT_RS34060at 3241.485 kb on + strand, within BPHYT_RS34060at 3241.485 kb on + strand, within BPHYT_RS34060at 3241.566 kb on + strand, within BPHYT_RS34060at 3241.671 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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3,239,912 - BPHYT_RS34050 0.28 +0.0
3,240,056 + BPHYT_RS34050 0.44 -1.6
3,240,125 + BPHYT_RS34050 0.52 -1.6
3,240,126 - BPHYT_RS34050 0.52 -0.6
3,240,305 - BPHYT_RS34050 0.72 -0.8
3,240,566 - +0.5
3,241,444 - BPHYT_RS34060 0.66 -0.2
3,241,485 + BPHYT_RS34060 0.71 -0.1
3,241,485 + BPHYT_RS34060 0.71 +0.7
3,241,566 + BPHYT_RS34060 0.80 -0.5
3,241,671 - -0.1

Or see this region's nucleotide sequence