Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27305

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27300 and BPHYT_RS27305 are separated by 338 nucleotidesBPHYT_RS27305 and BPHYT_RS27310 are separated by 445 nucleotides BPHYT_RS27300: BPHYT_RS27300 - hypothetical protein, at 1,701,021 to 1,701,809 _RS27300 BPHYT_RS27305: BPHYT_RS27305 - hypothetical protein, at 1,702,148 to 1,702,495 _RS27305 BPHYT_RS27310: BPHYT_RS27310 - LysR family transcriptional regulator, at 1,702,941 to 1,703,912 _RS27310 Position (kb) 1702 1703Strain fitness (log2 ratio) -1 0 1 2 3at 1701.222 kb on + strand, within BPHYT_RS27300at 1701.245 kb on + strand, within BPHYT_RS27300at 1701.511 kb on + strand, within BPHYT_RS27300at 1701.512 kb on - strand, within BPHYT_RS27300at 1702.030 kb on - strandat 1702.050 kb on - strandat 1702.274 kb on + strand, within BPHYT_RS27305at 1702.424 kb on + strand, within BPHYT_RS27305at 1702.715 kb on - strandat 1702.720 kb on - strandat 1702.865 kb on - strandat 1702.943 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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1,701,222 + BPHYT_RS27300 0.25 +0.5
1,701,245 + BPHYT_RS27300 0.28 -0.1
1,701,511 + BPHYT_RS27300 0.62 +3.0
1,701,512 - BPHYT_RS27300 0.62 +0.7
1,702,030 - +0.5
1,702,050 - -0.3
1,702,274 + BPHYT_RS27305 0.36 +1.2
1,702,424 + BPHYT_RS27305 0.79 +1.2
1,702,715 - -0.7
1,702,720 - +0.5
1,702,865 - -0.6
1,702,943 - +0.8

Or see this region's nucleotide sequence