Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25935

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25930 and BPHYT_RS25935 overlap by 4 nucleotidesBPHYT_RS25935 and BPHYT_RS25940 are separated by 235 nucleotidesBPHYT_RS25940 and BPHYT_RS25945 are separated by 227 nucleotides BPHYT_RS25930: BPHYT_RS25930 - hypothetical protein, at 1,393,232 to 1,394,332 _RS25930 BPHYT_RS25935: BPHYT_RS25935 - type III secretion protein, at 1,394,329 to 1,396,242 _RS25935 BPHYT_RS25940: BPHYT_RS25940 - arylsulfate sulfotransferase, at 1,396,478 to 1,396,732 _RS25940 BPHYT_RS25945: BPHYT_RS25945 - hypothetical protein, at 1,396,960 to 1,397,223 _RS25945 Position (kb) 1394 1395 1396 1397Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1394.624 kb on + strand, within BPHYT_RS25935at 1395.182 kb on - strand, within BPHYT_RS25935at 1395.927 kb on - strand, within BPHYT_RS25935at 1396.166 kb on - strandat 1396.966 kb on + strandat 1397.199 kb on + strandat 1397.200 kb on - strandat 1397.241 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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1,394,624 + BPHYT_RS25935 0.15 +3.8
1,395,182 - BPHYT_RS25935 0.45 -2.2
1,395,927 - BPHYT_RS25935 0.83 -0.5
1,396,166 - +0.5
1,396,966 + -0.3
1,397,199 + +1.5
1,397,200 - +0.1
1,397,241 + -1.5

Or see this region's nucleotide sequence