Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25015 and BPHYT_RS25020 are separated by 138 nucleotides BPHYT_RS25020 and BPHYT_RS25025 are separated by 305 nucleotides BPHYT_RS25025 and BPHYT_RS25030 are separated by 269 nucleotides
BPHYT_RS25015: BPHYT_RS25015 - XRE family transcriptional regulator, at 1,182,248 to 1,182,817
_RS25015
BPHYT_RS25020: BPHYT_RS25020 - phospholipid-binding protein, at 1,182,956 to 1,183,315
_RS25020
BPHYT_RS25025: BPHYT_RS25025 - acyl-CoA dehydrogenase, at 1,183,621 to 1,185,426
_RS25025
BPHYT_RS25030: BPHYT_RS25030 - gluconate 2-dehydrogenase, at 1,185,696 to 1,186,469
_RS25030
Position (kb) 1183
1184
1185
1186 Strain fitness (log2 ratio) -2
-1
0
1 at 1182.707 kb on + strand, within BPHYT_RS25015 at 1182.815 kb on + strand at 1182.815 kb on + strand at 1182.829 kb on + strand at 1183.248 kb on + strand, within BPHYT_RS25020 at 1183.414 kb on - strand at 1183.448 kb on - strand at 1183.834 kb on - strand, within BPHYT_RS25025 at 1184.521 kb on + strand, within BPHYT_RS25025 at 1184.558 kb on + strand, within BPHYT_RS25025 at 1185.047 kb on + strand, within BPHYT_RS25025 at 1185.134 kb on + strand, within BPHYT_RS25025 at 1185.564 kb on - strand at 1185.633 kb on - strand at 1185.633 kb on - strand at 1185.965 kb on + strand, within BPHYT_RS25030 at 1186.186 kb on - strand, within BPHYT_RS25030 at 1186.344 kb on - strand, within BPHYT_RS25030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,182,707 + BPHYT_RS25015 0.81 -0.4 1,182,815 + -2.2 1,182,815 + +0.5 1,182,829 + +0.2 1,183,248 + BPHYT_RS25020 0.81 -0.8 1,183,414 - -0.2 1,183,448 - -0.4 1,183,834 - BPHYT_RS25025 0.12 +0.8 1,184,521 + BPHYT_RS25025 0.50 +1.3 1,184,558 + BPHYT_RS25025 0.52 +0.9 1,185,047 + BPHYT_RS25025 0.79 +1.3 1,185,134 + BPHYT_RS25025 0.84 -0.8 1,185,564 - +1.3 1,185,633 - +0.1 1,185,633 - +0.6 1,185,965 + BPHYT_RS25030 0.35 +0.1 1,186,186 - BPHYT_RS25030 0.63 +0.3 1,186,344 - BPHYT_RS25030 0.84 +1.0
Or see this region's nucleotide sequence