Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24935 and BPHYT_RS24940 are separated by 97 nucleotides BPHYT_RS24940 and BPHYT_RS24945 are separated by 321 nucleotides BPHYT_RS24945 and BPHYT_RS24950 are separated by 96 nucleotides BPHYT_RS24950 and BPHYT_RS24955 are separated by 111 nucleotides
BPHYT_RS24935: BPHYT_RS24935 - cysteinyl-tRNA synthetase, at 1,165,995 to 1,167,383
_RS24935
BPHYT_RS24940: BPHYT_RS24940 - hypothetical protein, at 1,167,481 to 1,167,729
_RS24940
BPHYT_RS24945: BPHYT_RS24945 - alkylhydroperoxidase, at 1,168,051 to 1,168,443
_RS24945
BPHYT_RS24950: BPHYT_RS24950 - HAD family hydrolase, at 1,168,540 to 1,169,232
_RS24950
BPHYT_RS24955: BPHYT_RS24955 - cytochrome C peroxidase, at 1,169,344 to 1,170,786
_RS24955
Position (kb)
1168
1169 Strain fitness (log2 ratio)
-1
0
1 at 1167.355 kb on + strand at 1167.356 kb on - strand at 1167.381 kb on + strand at 1167.845 kb on - strand at 1168.162 kb on + strand, within BPHYT_RS24945 at 1168.163 kb on - strand, within BPHYT_RS24945 at 1168.261 kb on + strand, within BPHYT_RS24945 at 1168.262 kb on - strand, within BPHYT_RS24945
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,167,355 + -0.3 1,167,356 - +0.2 1,167,381 + +0.2 1,167,845 - -1.2 1,168,162 + BPHYT_RS24945 0.28 +1.0 1,168,163 - BPHYT_RS24945 0.28 -1.0 1,168,261 + BPHYT_RS24945 0.53 -1.1 1,168,262 - BPHYT_RS24945 0.54 -0.8
Or see this region's nucleotide sequence