Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24020 and BPHYT_RS24025 are separated by 180 nucleotides BPHYT_RS24025 and BPHYT_RS24030 are separated by 25 nucleotides BPHYT_RS24030 and BPHYT_RS24035 are separated by 55 nucleotides
BPHYT_RS24020: BPHYT_RS24020 - phenylalanine/histidine ammonia-lyase, at 953,797 to 955,446
_RS24020
BPHYT_RS24025: BPHYT_RS24025 - histidine utilization repressor, at 955,627 to 956,310
_RS24025
BPHYT_RS24030: BPHYT_RS24030 - histidine utilization repressor, at 956,336 to 957,139
_RS24030
BPHYT_RS24035: BPHYT_RS24035 - ATP-binding protein, at 957,195 to 958,064
_RS24035
Position (kb) 955
956
957 Strain fitness (log2 ratio) -1
0
1 at 954.940 kb on + strand, within BPHYT_RS24020 at 954.941 kb on - strand, within BPHYT_RS24020 at 955.560 kb on + strand at 955.560 kb on + strand at 955.636 kb on + strand at 956.027 kb on - strand, within BPHYT_RS24025 at 956.038 kb on + strand, within BPHYT_RS24025 at 956.104 kb on + strand, within BPHYT_RS24025 at 956.105 kb on - strand, within BPHYT_RS24025 at 956.105 kb on - strand, within BPHYT_RS24025 at 956.150 kb on - strand, within BPHYT_RS24025 at 956.150 kb on - strand, within BPHYT_RS24025 at 956.171 kb on - strand, within BPHYT_RS24025 at 956.634 kb on + strand, within BPHYT_RS24030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 954,940 + BPHYT_RS24020 0.69 -0.6 954,941 - BPHYT_RS24020 0.69 -0.6 955,560 + -0.8 955,560 + -0.9 955,636 + +0.9 956,027 - BPHYT_RS24025 0.58 -0.8 956,038 + BPHYT_RS24025 0.60 -0.1 956,104 + BPHYT_RS24025 0.70 -0.1 956,105 - BPHYT_RS24025 0.70 -1.3 956,105 - BPHYT_RS24025 0.70 -0.1 956,150 - BPHYT_RS24025 0.76 -0.0 956,150 - BPHYT_RS24025 0.76 -1.0 956,171 - BPHYT_RS24025 0.80 -0.3 956,634 + BPHYT_RS24030 0.37 -1.1
Or see this region's nucleotide sequence