Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21605 and BPHYT_RS21610 are separated by 188 nucleotides BPHYT_RS21610 and BPHYT_RS21615 are separated by 117 nucleotides BPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides
BPHYT_RS21605: BPHYT_RS21605 - NmrA family transcriptional regulator, at 403,391 to 404,143
_RS21605
BPHYT_RS21610: BPHYT_RS21610 - DNA-binding protein, at 404,332 to 405,861
_RS21610
BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653
_RS21615
BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117
_RS21620
Position (kb) 404
405
406 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 403.349 kb on + strand at 403.350 kb on - strand at 403.380 kb on + strand at 404.293 kb on + strand at 404.546 kb on - strand, within BPHYT_RS21610 at 404.546 kb on - strand, within BPHYT_RS21610 at 404.898 kb on + strand, within BPHYT_RS21610 at 404.899 kb on - strand, within BPHYT_RS21610 at 405.349 kb on + strand, within BPHYT_RS21610 at 405.397 kb on + strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.563 kb on + strand, within BPHYT_RS21610 at 405.872 kb on - strand at 405.872 kb on - strand at 405.997 kb on - strand at 405.997 kb on - strand at 406.184 kb on + strand, within BPHYT_RS21615 at 406.735 kb on + strand, within BPHYT_RS21620
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 403,349 + -0.5 403,350 - -0.6 403,380 + +3.2 404,293 + -0.4 404,546 - BPHYT_RS21610 0.14 +0.6 404,546 - BPHYT_RS21610 0.14 +0.1 404,898 + BPHYT_RS21610 0.37 +0.0 404,899 - BPHYT_RS21610 0.37 +0.8 405,349 + BPHYT_RS21610 0.66 -0.0 405,397 + BPHYT_RS21610 0.70 -0.3 405,548 - BPHYT_RS21610 0.79 -2.1 405,548 - BPHYT_RS21610 0.79 +0.3 405,548 - BPHYT_RS21610 0.79 -0.1 405,563 + BPHYT_RS21610 0.80 +0.0 405,872 - +0.0 405,872 - +1.6 405,997 - -1.8 405,997 - -1.0 406,184 + BPHYT_RS21615 0.30 -0.8 406,735 + BPHYT_RS21620 0.18 -1.2
Or see this region's nucleotide sequence