Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20235 and BPHYT_RS20240 overlap by 4 nucleotides BPHYT_RS20240 and BPHYT_RS20245 are separated by 5 nucleotides BPHYT_RS20245 and BPHYT_RS20250 are separated by 92 nucleotides BPHYT_RS20250 and BPHYT_RS20255 are separated by 101 nucleotides
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
BPHYT_RS20240: BPHYT_RS20240 - iron reductase, at 85,932 to 86,750
_RS20240
BPHYT_RS20245: BPHYT_RS20245 - iron ABC transporter substrate-binding protein, at 86,756 to 87,856
_RS20245
BPHYT_RS20250: BPHYT_RS20250 - dehydrogenase, at 87,949 to 88,134
_RS20250
BPHYT_RS20255: BPHYT_RS20255 - peptide ABC transporter ATP-binding protein, at 88,236 to 90,002
_RS20255
Position (kb) 86
87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 86.202 kb on + strand, within BPHYT_RS20240 at 86.203 kb on - strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.206 kb on - strand, within BPHYT_RS20240 at 86.478 kb on + strand, within BPHYT_RS20240 at 86.650 kb on + strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.655 kb on + strand, within BPHYT_RS20240 at 86.702 kb on - strand at 86.867 kb on + strand, within BPHYT_RS20245 at 87.089 kb on + strand, within BPHYT_RS20245 at 87.092 kb on + strand, within BPHYT_RS20245 at 87.275 kb on + strand, within BPHYT_RS20245 at 87.276 kb on - strand, within BPHYT_RS20245 at 87.593 kb on + strand, within BPHYT_RS20245 at 87.796 kb on + strand at 87.808 kb on + strand at 87.808 kb on + strand at 87.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 86,202 + BPHYT_RS20240 0.33 -0.0 86,203 - BPHYT_RS20240 0.33 -2.9 86,205 + BPHYT_RS20240 0.33 +0.0 86,205 + BPHYT_RS20240 0.33 -0.2 86,206 - BPHYT_RS20240 0.33 -0.6 86,478 + BPHYT_RS20240 0.67 +1.2 86,650 + BPHYT_RS20240 0.88 -0.0 86,651 - BPHYT_RS20240 0.88 -0.1 86,651 - BPHYT_RS20240 0.88 -0.4 86,651 - BPHYT_RS20240 0.88 +0.9 86,655 + BPHYT_RS20240 0.88 -0.7 86,702 - +0.0 86,867 + BPHYT_RS20245 0.10 +1.2 87,089 + BPHYT_RS20245 0.30 -0.9 87,092 + BPHYT_RS20245 0.31 -0.6 87,275 + BPHYT_RS20245 0.47 -0.8 87,276 - BPHYT_RS20245 0.47 -0.6 87,593 + BPHYT_RS20245 0.76 -1.9 87,796 + +0.8 87,808 + -0.1 87,808 + -0.1 87,808 + -3.0
Or see this region's nucleotide sequence