Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20065

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotidesBPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotidesBPHYT_RS20065 and BPHYT_RS20070 are separated by 81 nucleotides BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202 _RS20055 BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122 _RS20060 BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604 _RS20065 BPHYT_RS20070: BPHYT_RS20070 - hypothetical protein, at 45,686 to 47,119 _RS20070 Position (kb) 45 46Strain fitness (log2 ratio) -2 -1 0 1at 44.236 kb on + strandat 44.360 kb on - strandat 44.639 kb on - strand, within BPHYT_RS20060at 45.145 kb on + strandat 45.418 kb on - strand, within BPHYT_RS20065at 45.630 kb on + strandat 45.631 kb on - strandat 45.646 kb on - strandat 45.661 kb on - strandat 46.098 kb on - strand, within BPHYT_RS20070

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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44,236 + +0.4
44,360 - +0.5
44,639 - BPHYT_RS20060 0.30 +0.4
45,145 + +1.5
45,418 - BPHYT_RS20065 0.61 -1.9
45,630 + -0.2
45,631 - -0.7
45,646 - +0.3
45,661 - -1.5
46,098 - BPHYT_RS20070 0.29 +0.5

Or see this region's nucleotide sequence