Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14340 and BPHYT_RS14345 are separated by 100 nucleotides BPHYT_RS14345 and BPHYT_RS14350 are separated by 69 nucleotides BPHYT_RS14350 and BPHYT_RS14355 are separated by 15 nucleotides
BPHYT_RS14340: BPHYT_RS14340 - Fis family transcriptional regulator, at 3,273,972 to 3,275,354
_RS14340
BPHYT_RS14345: BPHYT_RS14345 - beta-hexosaminidase, at 3,275,455 to 3,276,483
_RS14345
BPHYT_RS14350: BPHYT_RS14350 - holo-[acyl-carrier-protein] synthase, at 3,276,553 to 3,276,975
_RS14350
BPHYT_RS14355: BPHYT_RS14355 - pyridoxine 5'-phosphate synthase, at 3,276,991 to 3,277,755
_RS14355
Position (kb)
3275
3276
3277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3275.061 kb on + strand, within BPHYT_RS14340 at 3275.062 kb on - strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.081 kb on - strand, within BPHYT_RS14340 at 3275.112 kb on + strand, within BPHYT_RS14340 at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.321 kb on - strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.353 kb on - strand at 3275.407 kb on + strand at 3275.407 kb on + strand at 3275.456 kb on + strand at 3275.495 kb on + strand at 3275.873 kb on + strand, within BPHYT_RS14345 at 3276.093 kb on - strand, within BPHYT_RS14345 at 3276.161 kb on + strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 3,274,779 - BPHYT_RS14340 0.58 +0.6 3,274,779 - BPHYT_RS14340 0.58 +2.6 3,274,779 - BPHYT_RS14340 0.58 +1.1 3,274,779 - BPHYT_RS14340 0.58 +1.3 3,274,779 - BPHYT_RS14340 0.58 +0.9 3,275,061 + BPHYT_RS14340 0.79 +0.6 3,275,062 - BPHYT_RS14340 0.79 +0.3 3,275,080 + BPHYT_RS14340 0.80 +0.4 3,275,080 + BPHYT_RS14340 0.80 +0.9 3,275,080 + BPHYT_RS14340 0.80 +0.8 3,275,080 + BPHYT_RS14340 0.80 +0.4 3,275,081 - BPHYT_RS14340 0.80 +0.7 3,275,112 + BPHYT_RS14340 0.82 +0.5 3,275,320 + +1.5 3,275,320 + +0.8 3,275,320 + +0.8 3,275,320 + +0.8 3,275,321 - +0.7 3,275,322 + +0.5 3,275,322 + +0.7 3,275,322 + +0.9 3,275,339 + +0.3 3,275,339 + -2.8 3,275,339 + +0.9 3,275,340 - +0.3 3,275,340 - +1.4 3,275,340 - +0.7 3,275,353 - -0.5 3,275,407 + -0.7 3,275,407 + +0.3 3,275,456 + -0.3 3,275,495 + +0.3 3,275,873 + BPHYT_RS14345 0.41 +1.1 3,276,093 - BPHYT_RS14345 0.62 +0.5 3,276,161 + BPHYT_RS14345 0.69 -2.1 3,276,162 - BPHYT_RS14345 0.69 -1.3 3,276,162 - BPHYT_RS14345 0.69 -0.3 3,276,162 - BPHYT_RS14345 0.69 +2.1 3,276,549 - +0.6
Or see this region's nucleotide sequence