Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11965 and BPHYT_RS11970 are separated by 69 nucleotides BPHYT_RS11970 and BPHYT_RS11975 are separated by 179 nucleotides BPHYT_RS11975 and BPHYT_RS11980 are separated by 701 nucleotides
BPHYT_RS11965: BPHYT_RS11965 - alkylhydroperoxidase, at 2,723,595 to 2,724,074
_RS11965
BPHYT_RS11970: BPHYT_RS11970 - cupin, at 2,724,144 to 2,724,551
_RS11970
BPHYT_RS11975: BPHYT_RS11975 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 2,724,731 to 2,725,939
_RS11975
BPHYT_RS11980: BPHYT_RS11980 - LysR family transcriptional regulator, at 2,726,641 to 2,727,678
_RS11980
Position (kb) 2724
2725
2726 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2723.916 kb on + strand, within BPHYT_RS11965 at 2724.482 kb on + strand, within BPHYT_RS11970 at 2724.719 kb on + strand at 2725.292 kb on - strand, within BPHYT_RS11975 at 2725.752 kb on - strand, within BPHYT_RS11975 at 2725.786 kb on + strand, within BPHYT_RS11975 at 2725.791 kb on - strand, within BPHYT_RS11975 at 2726.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 2,723,916 + BPHYT_RS11965 0.67 +0.4 2,724,482 + BPHYT_RS11970 0.83 -2.1 2,724,719 + +2.0 2,725,292 - BPHYT_RS11975 0.46 -1.2 2,725,752 - BPHYT_RS11975 0.84 -0.3 2,725,786 + BPHYT_RS11975 0.87 -0.2 2,725,791 - BPHYT_RS11975 0.88 +0.4 2,726,501 + -1.6
Or see this region's nucleotide sequence