Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10005

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10000 and BPHYT_RS10005 are separated by 200 nucleotidesBPHYT_RS10005 and BPHYT_RS10010 are separated by 113 nucleotidesBPHYT_RS10010 and BPHYT_RS10015 overlap by 4 nucleotides BPHYT_RS10000: BPHYT_RS10000 - glucoamylase, at 2,277,862 to 2,279,733 _RS10000 BPHYT_RS10005: BPHYT_RS10005 - hypothetical protein, at 2,279,934 to 2,280,305 _RS10005 BPHYT_RS10010: BPHYT_RS10010 - cupin, at 2,280,419 to 2,280,850 _RS10010 BPHYT_RS10015: BPHYT_RS10015 - NAD(P)H dehydrogenase (quinone), at 2,280,847 to 2,281,530 _RS10015 Position (kb) 2279 2280 2281Strain fitness (log2 ratio) -2 -1 0 1 2at 2278.966 kb on + strand, within BPHYT_RS10000at 2279.061 kb on + strand, within BPHYT_RS10000at 2279.504 kb on + strand, within BPHYT_RS10000at 2279.504 kb on + strand, within BPHYT_RS10000at 2279.504 kb on + strand, within BPHYT_RS10000at 2279.505 kb on - strand, within BPHYT_RS10000at 2279.505 kb on - strand, within BPHYT_RS10000at 2280.111 kb on + strand, within BPHYT_RS10005at 2280.111 kb on + strand, within BPHYT_RS10005at 2280.363 kb on - strandat 2280.369 kb on - strandat 2281.059 kb on + strand, within BPHYT_RS10015at 2281.060 kb on - strand, within BPHYT_RS10015at 2281.079 kb on + strand, within BPHYT_RS10015at 2281.179 kb on - strand, within BPHYT_RS10015at 2281.245 kb on - strand, within BPHYT_RS10015at 2281.245 kb on - strand, within BPHYT_RS10015at 2281.245 kb on - strand, within BPHYT_RS10015

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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2,278,966 + BPHYT_RS10000 0.59 -0.6
2,279,061 + BPHYT_RS10000 0.64 +1.4
2,279,504 + BPHYT_RS10000 0.88 -0.3
2,279,504 + BPHYT_RS10000 0.88 -0.5
2,279,504 + BPHYT_RS10000 0.88 -0.7
2,279,505 - BPHYT_RS10000 0.88 -0.2
2,279,505 - BPHYT_RS10000 0.88 +1.0
2,280,111 + BPHYT_RS10005 0.48 +0.7
2,280,111 + BPHYT_RS10005 0.48 -2.4
2,280,363 - +0.6
2,280,369 - +0.3
2,281,059 + BPHYT_RS10015 0.31 +0.6
2,281,060 - BPHYT_RS10015 0.31 +0.3
2,281,079 + BPHYT_RS10015 0.34 +1.4
2,281,179 - BPHYT_RS10015 0.49 -0.7
2,281,245 - BPHYT_RS10015 0.58 +1.9
2,281,245 - BPHYT_RS10015 0.58 -0.5
2,281,245 - BPHYT_RS10015 0.58 -1.3

Or see this region's nucleotide sequence