Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09290 and BPHYT_RS09295 are separated by 121 nucleotides BPHYT_RS09295 and BPHYT_RS09300 are separated by 46 nucleotides BPHYT_RS09300 and BPHYT_RS09305 are separated by 87 nucleotides BPHYT_RS09305 and BPHYT_RS09310 are separated by 159 nucleotides
BPHYT_RS09290: BPHYT_RS09290 - helicase, at 2,104,724 to 2,108,206
_RS09290
BPHYT_RS09295: BPHYT_RS09295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,108,328 to 2,109,044
_RS09295
BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570
_RS09300
BPHYT_RS09305: BPHYT_RS09305 - alkyl hydroperoxide reductase, at 2,109,658 to 2,110,206
_RS09305
BPHYT_RS09310: BPHYT_RS09310 - sensor histidine kinase, at 2,110,366 to 2,111,715
_RS09310
Position (kb)
2109
2110 Strain fitness (log2 ratio)
-1
0
1 at 2108.178 kb on + strand at 2108.206 kb on + strand at 2108.252 kb on - strand at 2108.282 kb on + strand at 2108.283 kb on - strand at 2108.288 kb on - strand at 2109.893 kb on + strand, within BPHYT_RS09305 at 2110.067 kb on + strand, within BPHYT_RS09305 at 2110.068 kb on - strand, within BPHYT_RS09305 at 2110.068 kb on - strand, within BPHYT_RS09305 at 2110.188 kb on + strand at 2110.276 kb on + strand at 2110.276 kb on + strand at 2110.276 kb on + strand at 2110.277 kb on - strand at 2110.277 kb on - strand at 2110.529 kb on + strand, within BPHYT_RS09310 at 2110.529 kb on + strand, within BPHYT_RS09310 at 2110.530 kb on - strand, within BPHYT_RS09310
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 2,108,178 + +0.8 2,108,206 + -0.0 2,108,252 - -0.2 2,108,282 + -0.5 2,108,283 - -1.6 2,108,288 - +0.4 2,109,893 + BPHYT_RS09305 0.43 -0.6 2,110,067 + BPHYT_RS09305 0.74 -0.9 2,110,068 - BPHYT_RS09305 0.75 -0.9 2,110,068 - BPHYT_RS09305 0.75 +0.3 2,110,188 + -0.4 2,110,276 + +0.3 2,110,276 + -0.5 2,110,276 + -1.6 2,110,277 - -0.1 2,110,277 - -0.3 2,110,529 + BPHYT_RS09310 0.12 -1.0 2,110,529 + BPHYT_RS09310 0.12 +0.2 2,110,530 - BPHYT_RS09310 0.12 +0.3
Or see this region's nucleotide sequence