Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08720 and BPHYT_RS08725 are separated by 60 nucleotides BPHYT_RS08725 and BPHYT_RS08730 are separated by 206 nucleotides BPHYT_RS08730 and BPHYT_RS08735 are separated by 170 nucleotides BPHYT_RS08735 and BPHYT_RS08740 are separated by 138 nucleotides
BPHYT_RS08720: BPHYT_RS08720 - membrane protein, at 1,979,774 to 1,980,400
_RS08720
BPHYT_RS08725: BPHYT_RS08725 - hypothetical protein, at 1,980,461 to 1,980,706
_RS08725
BPHYT_RS08730: BPHYT_RS08730 - hypothetical protein, at 1,980,913 to 1,981,125
_RS08730
BPHYT_RS08735: BPHYT_RS08735 - aldo-keto reductase, at 1,981,296 to 1,982,348
_RS08735
BPHYT_RS08740: BPHYT_RS08740 - membrane protein, at 1,982,487 to 1,983,770
_RS08740
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1 at 1980.366 kb on - strand at 1980.769 kb on - strand at 1980.819 kb on - strand at 1980.855 kb on - strand at 1980.855 kb on - strand at 1980.855 kb on - strand at 1981.034 kb on + strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.208 kb on - strand at 1981.498 kb on - strand, within BPHYT_RS08735 at 1981.599 kb on - strand, within BPHYT_RS08735 at 1981.767 kb on - strand, within BPHYT_RS08735 at 1981.767 kb on - strand, within BPHYT_RS08735 at 1981.951 kb on - strand, within BPHYT_RS08735 at 1982.041 kb on - strand, within BPHYT_RS08735 at 1982.136 kb on + strand, within BPHYT_RS08735 at 1982.557 kb on + strand at 1982.576 kb on + strand at 1982.577 kb on - strand at 1982.726 kb on - strand, within BPHYT_RS08740 at 1982.726 kb on - strand, within BPHYT_RS08740 at 1982.740 kb on - strand, within BPHYT_RS08740 at 1982.795 kb on + strand, within BPHYT_RS08740
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,980,366 - -1.4 1,980,769 - +0.2 1,980,819 - -1.2 1,980,855 - -1.3 1,980,855 - -1.9 1,980,855 - -0.5 1,981,034 + BPHYT_RS08730 0.57 -0.2 1,981,035 - BPHYT_RS08730 0.57 +0.1 1,981,035 - BPHYT_RS08730 0.57 -0.8 1,981,035 - BPHYT_RS08730 0.57 -0.1 1,981,208 - +0.4 1,981,498 - BPHYT_RS08735 0.19 -0.5 1,981,599 - BPHYT_RS08735 0.29 +1.3 1,981,767 - BPHYT_RS08735 0.45 +0.9 1,981,767 - BPHYT_RS08735 0.45 +1.6 1,981,951 - BPHYT_RS08735 0.62 -0.4 1,982,041 - BPHYT_RS08735 0.71 +0.3 1,982,136 + BPHYT_RS08735 0.80 +0.0 1,982,557 + -0.4 1,982,576 + +0.3 1,982,577 - +0.5 1,982,726 - BPHYT_RS08740 0.19 -1.3 1,982,726 - BPHYT_RS08740 0.19 -0.5 1,982,740 - BPHYT_RS08740 0.20 -1.3 1,982,795 + BPHYT_RS08740 0.24 -0.6
Or see this region's nucleotide sequence