Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08475 and BPHYT_RS08480 overlap by 4 nucleotides BPHYT_RS08480 and BPHYT_RS08485 are separated by 207 nucleotides BPHYT_RS08485 and BPHYT_RS08490 are separated by 36 nucleotides
BPHYT_RS08475: BPHYT_RS08475 - hypothetical protein, at 1,939,027 to 1,939,326
_RS08475
BPHYT_RS08480: BPHYT_RS08480 - transcriptional regulator, at 1,939,323 to 1,939,745
_RS08480
BPHYT_RS08485: BPHYT_RS08485 - undecaprenyl-phosphate glucose phosphotransferase, at 1,939,953 to 1,941,350
_RS08485
BPHYT_RS08490: BPHYT_RS08490 - Crp/Fnr family transcriptional regulator, at 1,941,387 to 1,942,121
_RS08490
Position (kb)
1939
1940
1941
1942 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1939.139 kb on - strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.189 kb on + strand, within BPHYT_RS08475 at 1939.243 kb on + strand, within BPHYT_RS08475 at 1939.244 kb on - strand, within BPHYT_RS08475 at 1939.275 kb on - strand, within BPHYT_RS08475 at 1939.291 kb on + strand, within BPHYT_RS08475 at 1939.426 kb on - strand, within BPHYT_RS08480 at 1939.627 kb on + strand, within BPHYT_RS08480 at 1939.892 kb on + strand at 1940.030 kb on + strand at 1940.038 kb on + strand at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.178 kb on + strand, within BPHYT_RS08485 at 1940.696 kb on + strand, within BPHYT_RS08485 at 1941.009 kb on - strand, within BPHYT_RS08485 at 1941.182 kb on - strand, within BPHYT_RS08485 at 1941.319 kb on - strand at 1941.737 kb on - strand, within BPHYT_RS08490 at 1942.308 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,939,139 - BPHYT_RS08475 0.37 -0.8 1,939,174 + BPHYT_RS08475 0.49 -0.5 1,939,174 + BPHYT_RS08475 0.49 -0.5 1,939,189 + BPHYT_RS08475 0.54 +2.6 1,939,243 + BPHYT_RS08475 0.72 -0.9 1,939,244 - BPHYT_RS08475 0.72 -0.6 1,939,275 - BPHYT_RS08475 0.83 -0.3 1,939,291 + BPHYT_RS08475 0.88 -0.5 1,939,426 - BPHYT_RS08480 0.24 +3.7 1,939,627 + BPHYT_RS08480 0.72 -0.5 1,939,892 + -0.1 1,940,030 + -0.1 1,940,038 + -0.8 1,940,153 + BPHYT_RS08485 0.14 -1.1 1,940,153 + BPHYT_RS08485 0.14 -1.0 1,940,178 + BPHYT_RS08485 0.16 -1.3 1,940,696 + BPHYT_RS08485 0.53 -0.2 1,941,009 - BPHYT_RS08485 0.76 +0.7 1,941,182 - BPHYT_RS08485 0.88 +0.0 1,941,319 - -1.0 1,941,737 - BPHYT_RS08490 0.48 -1.7 1,942,308 + -0.5
Or see this region's nucleotide sequence