Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08410 and BPHYT_RS08415 are separated by 30 nucleotides BPHYT_RS08415 and BPHYT_RS08420 are separated by 136 nucleotides BPHYT_RS08420 and BPHYT_RS08425 are separated by 129 nucleotides
BPHYT_RS08410: BPHYT_RS08410 - dehydrogenase, at 1,927,708 to 1,928,649
_RS08410
BPHYT_RS08415: BPHYT_RS08415 - hypothetical protein, at 1,928,680 to 1,929,063
_RS08415
BPHYT_RS08420: BPHYT_RS08420 - histidine kinase, at 1,929,200 to 1,929,637
_RS08420
BPHYT_RS08425: BPHYT_RS08425 - hypothetical protein, at 1,929,767 to 1,930,639
_RS08425
Position (kb) 1929
1930 Strain fitness (log2 ratio) -1
0
1 at 1928.593 kb on + strand at 1928.628 kb on + strand at 1928.898 kb on + strand, within BPHYT_RS08415 at 1928.929 kb on + strand, within BPHYT_RS08415 at 1928.948 kb on - strand, within BPHYT_RS08415 at 1929.294 kb on - strand, within BPHYT_RS08420 at 1929.889 kb on + strand, within BPHYT_RS08425 at 1929.928 kb on - strand, within BPHYT_RS08425 at 1930.020 kb on + strand, within BPHYT_RS08425 at 1930.020 kb on + strand, within BPHYT_RS08425 at 1930.021 kb on - strand at 1930.501 kb on + strand, within BPHYT_RS08425 at 1930.501 kb on + strand, within BPHYT_RS08425
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,928,593 + +1.1 1,928,628 + -1.6 1,928,898 + BPHYT_RS08415 0.57 +0.2 1,928,929 + BPHYT_RS08415 0.65 -0.1 1,928,948 - BPHYT_RS08415 0.70 -0.0 1,929,294 - BPHYT_RS08420 0.21 -1.1 1,929,889 + BPHYT_RS08425 0.14 -0.6 1,929,928 - BPHYT_RS08425 0.18 +0.2 1,930,020 + BPHYT_RS08425 0.29 -1.1 1,930,020 + BPHYT_RS08425 0.29 +0.1 1,930,021 - -0.8 1,930,501 + BPHYT_RS08425 0.84 -1.2 1,930,501 + BPHYT_RS08425 0.84 -0.0
Or see this region's nucleotide sequence