Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05820 and BPHYT_RS05825 are separated by 32 nucleotides BPHYT_RS05825 and BPHYT_RS05830 are separated by 165 nucleotides BPHYT_RS05830 and BPHYT_RS05835 are separated by 32 nucleotides BPHYT_RS05835 and BPHYT_RS05840 overlap by 13 nucleotides BPHYT_RS05840 and BPHYT_RS05845 overlap by 4 nucleotides
BPHYT_RS05820: BPHYT_RS05820 - hypothetical protein, at 1,325,632 to 1,327,530
_RS05820
BPHYT_RS05825: BPHYT_RS05825 - hypothetical protein, at 1,327,563 to 1,327,784
_RS05825
BPHYT_RS05830: BPHYT_RS05830 - hypothetical protein, at 1,327,950 to 1,328,408
_RS05830
BPHYT_RS05835: BPHYT_RS05835 - hypothetical protein, at 1,328,441 to 1,328,845
_RS05835
BPHYT_RS05840: BPHYT_RS05840 - hypothetical protein, at 1,328,833 to 1,329,240
_RS05840
BPHYT_RS05845: BPHYT_RS05845 - hypothetical protein, at 1,329,237 to 1,329,659
_RS05845
Position (kb) 1327
1328
1329 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1326.951 kb on + strand, within BPHYT_RS05820 at 1326.954 kb on + strand, within BPHYT_RS05820 at 1326.955 kb on - strand, within BPHYT_RS05820 at 1327.013 kb on - strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.021 kb on - strand, within BPHYT_RS05820 at 1327.096 kb on - strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.183 kb on + strand, within BPHYT_RS05820 at 1327.227 kb on - strand, within BPHYT_RS05820 at 1327.314 kb on + strand, within BPHYT_RS05820 at 1327.315 kb on - strand, within BPHYT_RS05820 at 1327.368 kb on + strand at 1327.490 kb on - strand at 1327.519 kb on + strand at 1327.693 kb on + strand, within BPHYT_RS05825 at 1327.694 kb on - strand, within BPHYT_RS05825 at 1327.898 kb on + strand at 1328.009 kb on - strand, within BPHYT_RS05830 at 1328.223 kb on + strand, within BPHYT_RS05830 at 1328.245 kb on + strand, within BPHYT_RS05830 at 1328.375 kb on - strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.472 kb on - strand at 1328.478 kb on - strand at 1328.489 kb on + strand, within BPHYT_RS05835 at 1328.646 kb on - strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.650 kb on - strand, within BPHYT_RS05835 at 1328.654 kb on + strand, within BPHYT_RS05835 at 1328.654 kb on + strand, within BPHYT_RS05835 at 1328.685 kb on - strand, within BPHYT_RS05835 at 1328.705 kb on + strand, within BPHYT_RS05835 at 1328.876 kb on + strand, within BPHYT_RS05840 at 1328.877 kb on - strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 1,326,951 + BPHYT_RS05820 0.69 +0.9 1,326,954 + BPHYT_RS05820 0.70 +0.5 1,326,955 - BPHYT_RS05820 0.70 -0.1 1,327,013 - BPHYT_RS05820 0.73 +0.2 1,327,020 + BPHYT_RS05820 0.73 -1.0 1,327,020 + BPHYT_RS05820 0.73 -0.4 1,327,021 - BPHYT_RS05820 0.73 +0.0 1,327,096 - BPHYT_RS05820 0.77 -0.4 1,327,147 + BPHYT_RS05820 0.80 -0.5 1,327,147 + BPHYT_RS05820 0.80 +2.7 1,327,183 + BPHYT_RS05820 0.82 -0.7 1,327,227 - BPHYT_RS05820 0.84 -1.9 1,327,314 + BPHYT_RS05820 0.89 +0.6 1,327,315 - BPHYT_RS05820 0.89 +0.4 1,327,368 + +0.4 1,327,490 - -0.6 1,327,519 + +0.0 1,327,693 + BPHYT_RS05825 0.59 -1.6 1,327,694 - BPHYT_RS05825 0.59 -0.9 1,327,898 + -0.3 1,328,009 - BPHYT_RS05830 0.13 -0.1 1,328,223 + BPHYT_RS05830 0.59 -1.0 1,328,245 + BPHYT_RS05830 0.64 -0.8 1,328,375 - +0.7 1,328,471 + -0.0 1,328,471 + +0.9 1,328,471 + -1.0 1,328,471 + -0.2 1,328,472 - -1.4 1,328,478 - +1.9 1,328,489 + BPHYT_RS05835 0.12 +0.1 1,328,646 - BPHYT_RS05835 0.51 +0.5 1,328,649 + BPHYT_RS05835 0.51 +0.5 1,328,649 + BPHYT_RS05835 0.51 +3.9 1,328,649 + BPHYT_RS05835 0.51 +0.5 1,328,650 - BPHYT_RS05835 0.52 -0.5 1,328,654 + BPHYT_RS05835 0.53 -2.1 1,328,654 + BPHYT_RS05835 0.53 -0.2 1,328,685 - BPHYT_RS05835 0.60 -0.5 1,328,705 + BPHYT_RS05835 0.65 +0.5 1,328,876 + BPHYT_RS05840 0.11 +0.5 1,328,877 - BPHYT_RS05840 0.11 +1.4 1,328,893 + BPHYT_RS05840 0.15 +0.0 1,328,893 + BPHYT_RS05840 0.15 +0.5 1,328,893 + BPHYT_RS05840 0.15 +0.9
Or see this region's nucleotide sequence