Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05450

Experiment: 36hr PHYTO10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05445 and BPHYT_RS05450 are separated by 841 nucleotidesBPHYT_RS05450 and BPHYT_RS05455 are separated by 347 nucleotides BPHYT_RS05445: BPHYT_RS05445 - hypothetical protein, at 1,250,649 to 1,250,831 _RS05445 BPHYT_RS05450: BPHYT_RS05450 - AraC family transcriptional regulator, at 1,251,673 to 1,252,074 _RS05450 BPHYT_RS05455: BPHYT_RS05455 - AraC family transcriptional regulator, at 1,252,422 to 1,253,267 _RS05455 Position (kb) 1251 1252 1253Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1250.872 kb on + strandat 1251.470 kb on + strandat 1251.517 kb on + strandat 1251.610 kb on - strandat 1251.892 kb on + strand, within BPHYT_RS05450at 1252.154 kb on - strandat 1252.232 kb on + strandat 1252.635 kb on + strand, within BPHYT_RS05455at 1252.635 kb on + strand, within BPHYT_RS05455at 1252.636 kb on - strand, within BPHYT_RS05455at 1252.766 kb on + strand, within BPHYT_RS05455

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1
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1,250,872 + +0.3
1,251,470 + -0.2
1,251,517 + +1.5
1,251,610 - +1.5
1,251,892 + BPHYT_RS05450 0.54 -0.5
1,252,154 - -0.3
1,252,232 + +0.0
1,252,635 + BPHYT_RS05455 0.25 -0.5
1,252,635 + BPHYT_RS05455 0.25 -2.4
1,252,636 - BPHYT_RS05455 0.25 +3.1
1,252,766 + BPHYT_RS05455 0.41 -1.1

Or see this region's nucleotide sequence