Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04060 and BPHYT_RS04065 are separated by 59 nucleotides BPHYT_RS04065 and BPHYT_RS04070 are separated by 110 nucleotides BPHYT_RS04070 and BPHYT_RS04075 are separated by 467 nucleotides
BPHYT_RS04060: BPHYT_RS04060 - glycosyl transferase, at 926,489 to 927,562
_RS04060
BPHYT_RS04065: BPHYT_RS04065 - hypothetical protein, at 927,622 to 928,833
_RS04065
BPHYT_RS04070: BPHYT_RS04070 - hypothetical protein, at 928,944 to 929,357
_RS04070
BPHYT_RS04075: BPHYT_RS04075 - hypothetical protein, at 929,825 to 930,220
_RS04075
Position (kb) 927
928
929 Strain fitness (log2 ratio) -2
-1
0
1 at 926.813 kb on + strand, within BPHYT_RS04060 at 927.116 kb on + strand, within BPHYT_RS04060 at 927.117 kb on - strand, within BPHYT_RS04060 at 927.144 kb on - strand, within BPHYT_RS04060 at 927.646 kb on + strand at 927.646 kb on + strand at 927.646 kb on + strand at 927.647 kb on - strand at 927.804 kb on + strand, within BPHYT_RS04065 at 927.804 kb on + strand, within BPHYT_RS04065 at 927.805 kb on - strand, within BPHYT_RS04065 at 928.475 kb on + strand, within BPHYT_RS04065 at 928.926 kb on - strand at 929.483 kb on - strand at 929.506 kb on + strand at 929.507 kb on - strand at 929.587 kb on - strand at 929.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 926,813 + BPHYT_RS04060 0.30 -0.3 927,116 + BPHYT_RS04060 0.58 +0.5 927,117 - BPHYT_RS04060 0.58 -1.0 927,144 - BPHYT_RS04060 0.61 +1.5 927,646 + -0.3 927,646 + -0.5 927,646 + +0.1 927,647 - -0.2 927,804 + BPHYT_RS04065 0.15 -2.2 927,804 + BPHYT_RS04065 0.15 -0.7 927,805 - BPHYT_RS04065 0.15 -1.6 928,475 + BPHYT_RS04065 0.70 -1.6 928,926 - +0.4 929,483 - +1.1 929,506 + -0.6 929,507 - +0.5 929,587 - -1.4 929,682 + -0.0
Or see this region's nucleotide sequence