Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02290 and BPHYT_RS02295 are separated by 442 nucleotides BPHYT_RS02295 and BPHYT_RS02300 are separated by 142 nucleotides BPHYT_RS02300 and BPHYT_RS02305 are separated by 159 nucleotides
BPHYT_RS02290: BPHYT_RS02290 - arginine--tRNA ligase, at 524,071 to 525,861
_RS02290
BPHYT_RS02295: BPHYT_RS02295 - hypothetical protein, at 526,304 to 526,630
_RS02295
BPHYT_RS02300: BPHYT_RS02300 - acid shock protein, at 526,773 to 526,982
_RS02300
BPHYT_RS02305: BPHYT_RS02305 - methionine synthase, at 527,142 to 529,850
_RS02305
Position (kb) 526
527 Strain fitness (log2 ratio) -2
-1
0
1
2 at 526.075 kb on - strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.176 kb on - strand at 526.176 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.368 kb on - strand, within BPHYT_RS02295 at 526.719 kb on + strand at 526.720 kb on - strand at 526.775 kb on - strand at 526.877 kb on - strand, within BPHYT_RS02300 at 527.011 kb on - strand at 527.011 kb on - strand at 527.063 kb on - strand at 527.091 kb on + strand at 527.143 kb on + strand at 527.203 kb on + strand at 527.204 kb on - strand at 527.245 kb on + strand at 527.245 kb on + strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.419 kb on + strand, within BPHYT_RS02305 at 527.419 kb on + strand, within BPHYT_RS02305 at 527.560 kb on + strand, within BPHYT_RS02305 at 527.638 kb on + strand, within BPHYT_RS02305 at 527.639 kb on - strand, within BPHYT_RS02305 at 527.782 kb on + strand, within BPHYT_RS02305
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 526,075 - -0.4 526,172 + +0.1 526,172 + -0.6 526,172 + +0.3 526,172 + -0.4 526,172 + -0.6 526,173 - +0.1 526,173 - -1.2 526,173 - +2.0 526,173 - -1.4 526,175 + +1.1 526,175 + -0.7 526,175 + +0.9 526,175 + -1.1 526,175 + +0.5 526,175 + -0.3 526,176 - -1.0 526,176 - -0.3 526,313 - -0.3 526,313 - -0.8 526,313 - -0.5 526,367 + BPHYT_RS02295 0.19 -0.2 526,367 + BPHYT_RS02295 0.19 -1.1 526,367 + BPHYT_RS02295 0.19 -0.9 526,368 - BPHYT_RS02295 0.20 -1.8 526,719 + +0.1 526,720 - +0.2 526,775 - +0.1 526,877 - BPHYT_RS02300 0.50 -1.1 527,011 - +0.6 527,011 - +0.2 527,063 - -0.3 527,091 + +0.2 527,143 + -1.5 527,203 + +0.5 527,204 - -0.7 527,245 + -1.8 527,245 + +0.6 527,246 - -1.2 527,246 - +0.0 527,246 - -0.5 527,246 - -1.3 527,419 + BPHYT_RS02305 0.10 +0.3 527,419 + BPHYT_RS02305 0.10 -0.6 527,560 + BPHYT_RS02305 0.15 +0.5 527,638 + BPHYT_RS02305 0.18 +0.1 527,639 - BPHYT_RS02305 0.18 +0.5 527,782 + BPHYT_RS02305 0.24 -0.3
Or see this region's nucleotide sequence