Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01500 and BPHYT_RS01505 are separated by 62 nucleotides BPHYT_RS01505 and BPHYT_RS01510 are separated by 6 nucleotides BPHYT_RS01510 and BPHYT_RS01515 are separated by 213 nucleotides BPHYT_RS01515 and BPHYT_RS01520 overlap by 4 nucleotides
BPHYT_RS01500: BPHYT_RS01500 - 7-cyano-7-deazaguanine synthase, at 342,450 to 343,184
_RS01500
BPHYT_RS01505: BPHYT_RS01505 - 7-cyano-7-deazaguanine reductase, at 343,247 to 343,879
_RS01505
BPHYT_RS01510: BPHYT_RS01510 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 343,886 to 344,332
_RS01510
BPHYT_RS01515: BPHYT_RS01515 - 2-dehydro-3-deoxyglucarate aldolase, at 344,546 to 345,328
_RS01515
BPHYT_RS01520: BPHYT_RS01520 - hypothetical protein, at 345,325 to 346,029
_RS01520
Position (kb) 343
344
345 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 342.935 kb on + strand, within BPHYT_RS01500 at 342.935 kb on + strand, within BPHYT_RS01500 at 342.936 kb on - strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.049 kb on + strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.050 kb on - strand, within BPHYT_RS01500 at 343.056 kb on + strand, within BPHYT_RS01500 at 343.056 kb on + strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.057 kb on - strand, within BPHYT_RS01500 at 343.146 kb on + strand at 343.146 kb on + strand at 343.146 kb on + strand at 343.146 kb on + strand at 343.146 kb on + strand at 343.146 kb on + strand at 343.147 kb on - strand at 343.253 kb on + strand at 343.275 kb on - strand at 343.436 kb on + strand, within BPHYT_RS01505 at 343.437 kb on - strand, within BPHYT_RS01505 at 343.731 kb on - strand, within BPHYT_RS01505 at 343.862 kb on + strand at 343.862 kb on + strand at 343.862 kb on + strand at 343.862 kb on + strand at 343.862 kb on + strand at 343.863 kb on - strand at 343.957 kb on + strand, within BPHYT_RS01510 at 343.958 kb on - strand, within BPHYT_RS01510 at 343.976 kb on + strand, within BPHYT_RS01510 at 343.976 kb on + strand, within BPHYT_RS01510 at 343.976 kb on + strand, within BPHYT_RS01510 at 343.976 kb on + strand, within BPHYT_RS01510 at 343.977 kb on - strand, within BPHYT_RS01510 at 343.977 kb on - strand, within BPHYT_RS01510 at 343.977 kb on - strand, within BPHYT_RS01510 at 343.977 kb on - strand, within BPHYT_RS01510 at 343.977 kb on - strand, within BPHYT_RS01510 at 344.117 kb on + strand, within BPHYT_RS01510 at 344.117 kb on + strand, within BPHYT_RS01510 at 344.118 kb on - strand, within BPHYT_RS01510 at 344.118 kb on - strand, within BPHYT_RS01510 at 344.128 kb on + strand, within BPHYT_RS01510 at 344.129 kb on - strand, within BPHYT_RS01510 at 344.129 kb on - strand, within BPHYT_RS01510 at 344.330 kb on + strand at 344.330 kb on + strand at 344.331 kb on - strand at 344.339 kb on + strand at 344.371 kb on + strand at 344.382 kb on + strand at 344.767 kb on - strand, within BPHYT_RS01515 at 344.767 kb on - strand, within BPHYT_RS01515 at 344.767 kb on - strand, within BPHYT_RS01515 at 344.808 kb on + strand, within BPHYT_RS01515 at 344.808 kb on + strand, within BPHYT_RS01515 at 344.808 kb on + strand, within BPHYT_RS01515 at 344.969 kb on + strand, within BPHYT_RS01515 at 344.970 kb on - strand, within BPHYT_RS01515 at 345.200 kb on - strand, within BPHYT_RS01515 at 345.318 kb on + strand at 345.318 kb on + strand at 345.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 342,935 + BPHYT_RS01500 0.66 +0.3 342,935 + BPHYT_RS01500 0.66 -0.6 342,936 - BPHYT_RS01500 0.66 +0.4 343,049 + BPHYT_RS01500 0.81 -0.8 343,049 + BPHYT_RS01500 0.81 +0.2 343,049 + BPHYT_RS01500 0.81 -0.6 343,049 + BPHYT_RS01500 0.81 -0.2 343,049 + BPHYT_RS01500 0.81 +0.4 343,049 + BPHYT_RS01500 0.81 -0.1 343,049 + BPHYT_RS01500 0.81 +0.1 343,050 - BPHYT_RS01500 0.82 +0.6 343,050 - BPHYT_RS01500 0.82 -1.8 343,050 - BPHYT_RS01500 0.82 -1.8 343,050 - BPHYT_RS01500 0.82 -0.4 343,050 - BPHYT_RS01500 0.82 -0.1 343,050 - BPHYT_RS01500 0.82 -0.2 343,050 - BPHYT_RS01500 0.82 +0.4 343,056 + BPHYT_RS01500 0.82 +0.4 343,056 + BPHYT_RS01500 0.82 -0.9 343,057 - BPHYT_RS01500 0.83 -0.5 343,057 - BPHYT_RS01500 0.83 +1.0 343,057 - BPHYT_RS01500 0.83 +0.4 343,057 - BPHYT_RS01500 0.83 -0.2 343,057 - BPHYT_RS01500 0.83 +0.1 343,057 - BPHYT_RS01500 0.83 +0.5 343,146 + +0.4 343,146 + +0.2 343,146 + +0.4 343,146 + -0.3 343,146 + +0.7 343,146 + -0.1 343,147 - -0.9 343,253 + +1.1 343,275 - +0.2 343,436 + BPHYT_RS01505 0.30 -1.1 343,437 - BPHYT_RS01505 0.30 +0.1 343,731 - BPHYT_RS01505 0.76 -1.1 343,862 + -1.8 343,862 + +0.7 343,862 + +0.2 343,862 + +0.1 343,862 + +2.8 343,863 - +0.6 343,957 + BPHYT_RS01510 0.16 +0.3 343,958 - BPHYT_RS01510 0.16 +0.6 343,976 + BPHYT_RS01510 0.20 -1.0 343,976 + BPHYT_RS01510 0.20 +0.0 343,976 + BPHYT_RS01510 0.20 -1.0 343,976 + BPHYT_RS01510 0.20 -0.2 343,977 - BPHYT_RS01510 0.20 -0.0 343,977 - BPHYT_RS01510 0.20 -0.2 343,977 - BPHYT_RS01510 0.20 -1.6 343,977 - BPHYT_RS01510 0.20 -0.9 343,977 - BPHYT_RS01510 0.20 +0.9 344,117 + BPHYT_RS01510 0.52 +0.5 344,117 + BPHYT_RS01510 0.52 +0.7 344,118 - BPHYT_RS01510 0.52 -0.4 344,118 - BPHYT_RS01510 0.52 -2.5 344,128 + BPHYT_RS01510 0.54 +0.3 344,129 - BPHYT_RS01510 0.54 -1.2 344,129 - BPHYT_RS01510 0.54 -0.2 344,330 + -0.3 344,330 + +0.2 344,331 - -1.6 344,339 + -0.2 344,371 + +0.3 344,382 + +0.2 344,767 - BPHYT_RS01515 0.28 +1.9 344,767 - BPHYT_RS01515 0.28 -0.1 344,767 - BPHYT_RS01515 0.28 +0.3 344,808 + BPHYT_RS01515 0.33 +0.2 344,808 + BPHYT_RS01515 0.33 -0.1 344,808 + BPHYT_RS01515 0.33 +0.5 344,969 + BPHYT_RS01515 0.54 +1.1 344,970 - BPHYT_RS01515 0.54 +0.1 345,200 - BPHYT_RS01515 0.84 -0.3 345,318 + -0.9 345,318 + -2.0 345,319 - -0.3
Or see this region's nucleotide sequence