Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01080 and BPHYT_RS01085 are separated by 689 nucleotides
BPHYT_RS01080: BPHYT_RS01080 - hypothetical protein, at 249,234 to 250,370
_RS01080
BPHYT_RS01085: BPHYT_RS01085 - transposase IS116/IS110/IS902 family protein, at 251,060 to 252,160
_RS01085
Position (kb) 249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 248.287 kb on + strand at 248.288 kb on - strand at 248.392 kb on - strand at 248.742 kb on + strand at 248.742 kb on + strand at 248.801 kb on - strand at 249.022 kb on + strand at 249.023 kb on - strand at 249.146 kb on + strand at 249.202 kb on - strand at 249.262 kb on + strand at 249.263 kb on - strand at 249.344 kb on + strand at 249.374 kb on - strand, within BPHYT_RS01080 at 249.403 kb on + strand, within BPHYT_RS01080 at 249.415 kb on + strand, within BPHYT_RS01080 at 249.416 kb on - strand, within BPHYT_RS01080 at 249.588 kb on + strand, within BPHYT_RS01080 at 249.590 kb on + strand, within BPHYT_RS01080 at 249.634 kb on - strand, within BPHYT_RS01080 at 249.656 kb on - strand, within BPHYT_RS01080 at 249.656 kb on - strand, within BPHYT_RS01080 at 249.687 kb on + strand, within BPHYT_RS01080 at 249.688 kb on - strand, within BPHYT_RS01080 at 249.897 kb on + strand, within BPHYT_RS01080 at 249.898 kb on - strand, within BPHYT_RS01080 at 249.954 kb on + strand, within BPHYT_RS01080 at 249.954 kb on + strand, within BPHYT_RS01080 at 249.998 kb on - strand, within BPHYT_RS01080 at 250.048 kb on - strand, within BPHYT_RS01080 at 250.118 kb on + strand, within BPHYT_RS01080 at 250.253 kb on + strand, within BPHYT_RS01080 at 250.253 kb on + strand, within BPHYT_RS01080 at 250.253 kb on + strand, within BPHYT_RS01080 at 250.253 kb on + strand, within BPHYT_RS01080 at 250.274 kb on + strand at 250.274 kb on + strand at 250.274 kb on + strand at 250.334 kb on - strand at 250.334 kb on - strand at 250.336 kb on - strand at 250.643 kb on - strand at 250.684 kb on + strand at 250.731 kb on + strand at 250.751 kb on - strand at 250.898 kb on - strand at 251.049 kb on + strand at 251.105 kb on + strand at 251.105 kb on + strand at 251.105 kb on + strand at 251.106 kb on - strand at 251.215 kb on + strand, within BPHYT_RS01085 at 251.215 kb on + strand, within BPHYT_RS01085 at 251.246 kb on + strand, within BPHYT_RS01085 at 251.247 kb on - strand, within BPHYT_RS01085 at 251.300 kb on + strand, within BPHYT_RS01085 at 251.301 kb on - strand, within BPHYT_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 248,287 + -0.2 248,288 - +0.7 248,392 - -0.3 248,742 + -0.9 248,742 + +1.3 248,801 - -0.9 249,022 + +0.7 249,023 - +1.5 249,146 + +1.0 249,202 - -0.4 249,262 + +0.5 249,263 - +1.0 249,344 + -1.5 249,374 - BPHYT_RS01080 0.12 -1.3 249,403 + BPHYT_RS01080 0.15 -0.8 249,415 + BPHYT_RS01080 0.16 -1.2 249,416 - BPHYT_RS01080 0.16 +0.2 249,588 + BPHYT_RS01080 0.31 -0.7 249,590 + BPHYT_RS01080 0.31 -0.2 249,634 - BPHYT_RS01080 0.35 -0.3 249,656 - BPHYT_RS01080 0.37 +1.4 249,656 - BPHYT_RS01080 0.37 +1.0 249,687 + BPHYT_RS01080 0.40 +0.7 249,688 - BPHYT_RS01080 0.40 -0.1 249,897 + BPHYT_RS01080 0.58 +0.2 249,898 - BPHYT_RS01080 0.58 -0.4 249,954 + BPHYT_RS01080 0.63 -1.1 249,954 + BPHYT_RS01080 0.63 -1.0 249,998 - BPHYT_RS01080 0.67 -0.3 250,048 - BPHYT_RS01080 0.72 -2.2 250,118 + BPHYT_RS01080 0.78 +1.0 250,253 + BPHYT_RS01080 0.90 -1.3 250,253 + BPHYT_RS01080 0.90 -0.9 250,253 + BPHYT_RS01080 0.90 +0.6 250,253 + BPHYT_RS01080 0.90 -0.1 250,274 + +0.1 250,274 + -1.5 250,274 + -0.4 250,334 - -0.5 250,334 - -0.4 250,336 - -2.0 250,643 - -0.2 250,684 + -0.6 250,731 + +1.0 250,751 - +1.3 250,898 - -0.8 251,049 + +0.5 251,105 + -1.6 251,105 + +2.1 251,105 + -2.8 251,106 - -0.4 251,215 + BPHYT_RS01085 0.14 -0.3 251,215 + BPHYT_RS01085 0.14 +0.4 251,246 + BPHYT_RS01085 0.17 +0.9 251,247 - BPHYT_RS01085 0.17 +0.4 251,300 + BPHYT_RS01085 0.22 +0.5 251,301 - BPHYT_RS01085 0.22 -0.7
Or see this region's nucleotide sequence