Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01060 and BPHYT_RS01065 are separated by 161 nucleotides BPHYT_RS01065 and BPHYT_RS01070 are separated by 48 nucleotides
BPHYT_RS01060: BPHYT_RS01060 - alcohol dehydrogenase, at 241,745 to 242,998
_RS01060
BPHYT_RS01065: BPHYT_RS01065 - GMC family oxidoreductase, at 243,160 to 244,938
_RS01065
BPHYT_RS01070: BPHYT_RS01070 - gluconate 2-dehydrogenase, at 244,987 to 245,706
_RS01070
Position (kb) 243
244
245 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.557 kb on + strand, within BPHYT_RS01060 at 242.602 kb on - strand, within BPHYT_RS01060 at 242.605 kb on + strand, within BPHYT_RS01060 at 242.862 kb on + strand, within BPHYT_RS01060 at 242.863 kb on - strand, within BPHYT_RS01060 at 242.885 kb on - strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.140 kb on - strand at 243.140 kb on - strand at 243.195 kb on - strand at 243.224 kb on + strand at 243.257 kb on + strand at 243.510 kb on - strand, within BPHYT_RS01065 at 243.602 kb on + strand, within BPHYT_RS01065 at 243.647 kb on + strand, within BPHYT_RS01065 at 243.648 kb on - strand, within BPHYT_RS01065 at 243.753 kb on - strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.873 kb on - strand, within BPHYT_RS01065 at 243.947 kb on + strand, within BPHYT_RS01065 at 243.948 kb on - strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.059 kb on - strand, within BPHYT_RS01065 at 244.059 kb on - strand, within BPHYT_RS01065 at 244.253 kb on + strand, within BPHYT_RS01065 at 244.514 kb on + strand, within BPHYT_RS01065 at 244.514 kb on + strand, within BPHYT_RS01065 at 244.670 kb on + strand, within BPHYT_RS01065 at 244.764 kb on - strand at 244.810 kb on - strand at 245.324 kb on + strand, within BPHYT_RS01070 at 245.325 kb on - strand, within BPHYT_RS01070 at 245.325 kb on - strand, within BPHYT_RS01070 at 245.342 kb on + strand, within BPHYT_RS01070 at 245.343 kb on - strand, within BPHYT_RS01070 at 245.420 kb on + strand, within BPHYT_RS01070 at 245.791 kb on + strand at 245.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 242,367 + BPHYT_RS01060 0.50 -1.5 242,367 + BPHYT_RS01060 0.50 +0.0 242,367 + BPHYT_RS01060 0.50 -0.4 242,557 + BPHYT_RS01060 0.65 +0.7 242,602 - BPHYT_RS01060 0.68 +0.2 242,605 + BPHYT_RS01060 0.69 -0.5 242,862 + BPHYT_RS01060 0.89 -0.8 242,863 - BPHYT_RS01060 0.89 -0.2 242,885 - -0.2 243,139 + -1.5 243,139 + -0.2 243,139 + -0.1 243,140 - -0.8 243,140 - -0.1 243,195 - -0.5 243,224 + -1.0 243,257 + -0.5 243,510 - BPHYT_RS01065 0.20 -0.6 243,602 + BPHYT_RS01065 0.25 +0.5 243,647 + BPHYT_RS01065 0.27 +0.4 243,648 - BPHYT_RS01065 0.27 -1.6 243,753 - BPHYT_RS01065 0.33 +0.5 243,872 + BPHYT_RS01065 0.40 +2.7 243,872 + BPHYT_RS01065 0.40 -0.3 243,872 + BPHYT_RS01065 0.40 +1.4 243,873 - BPHYT_RS01065 0.40 -0.5 243,947 + BPHYT_RS01065 0.44 +0.8 243,948 - BPHYT_RS01065 0.44 +1.1 244,058 + BPHYT_RS01065 0.50 +0.5 244,058 + BPHYT_RS01065 0.50 -0.1 244,058 + BPHYT_RS01065 0.50 +0.6 244,059 - BPHYT_RS01065 0.51 -0.2 244,059 - BPHYT_RS01065 0.51 -0.2 244,253 + BPHYT_RS01065 0.61 +4.3 244,514 + BPHYT_RS01065 0.76 -0.2 244,514 + BPHYT_RS01065 0.76 -0.1 244,670 + BPHYT_RS01065 0.85 +1.1 244,764 - -0.6 244,810 - +0.5 245,324 + BPHYT_RS01070 0.47 -1.5 245,325 - BPHYT_RS01070 0.47 +1.3 245,325 - BPHYT_RS01070 0.47 -0.1 245,342 + BPHYT_RS01070 0.49 +1.1 245,343 - BPHYT_RS01070 0.49 +0.6 245,420 + BPHYT_RS01070 0.60 +0.6 245,791 + +0.5 245,888 - +1.3
Or see this region's nucleotide sequence