Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00375 and BPHYT_RS00380 are separated by 134 nucleotides BPHYT_RS00380 and BPHYT_RS00385 overlap by 4 nucleotides
BPHYT_RS00375: BPHYT_RS00375 - sugar ABC transporter, at 94,579 to 95,499
_RS00375
BPHYT_RS00380: BPHYT_RS00380 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 95,634 to 96,227
_RS00380
BPHYT_RS00385: BPHYT_RS00385 - decarboxylase, at 96,224 to 97,567
_RS00385
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 94.681 kb on - strand, within BPHYT_RS00375 at 94.874 kb on + strand, within BPHYT_RS00375 at 95.133 kb on + strand, within BPHYT_RS00375 at 95.221 kb on - strand, within BPHYT_RS00375 at 95.397 kb on + strand, within BPHYT_RS00375 at 95.398 kb on - strand, within BPHYT_RS00375 at 95.405 kb on + strand, within BPHYT_RS00375 at 95.406 kb on - strand, within BPHYT_RS00375 at 95.504 kb on - strand at 95.704 kb on - strand, within BPHYT_RS00380 at 95.965 kb on - strand, within BPHYT_RS00380 at 95.965 kb on - strand, within BPHYT_RS00380 at 96.164 kb on - strand, within BPHYT_RS00380 at 96.382 kb on - strand, within BPHYT_RS00385 at 96.382 kb on - strand, within BPHYT_RS00385 at 96.419 kb on - strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.469 kb on - strand, within BPHYT_RS00385 at 96.469 kb on - strand, within BPHYT_RS00385 at 96.510 kb on + strand, within BPHYT_RS00385 at 96.565 kb on - strand, within BPHYT_RS00385 at 96.589 kb on + strand, within BPHYT_RS00385 at 96.590 kb on - strand, within BPHYT_RS00385 at 96.623 kb on + strand, within BPHYT_RS00385 at 96.623 kb on + strand, within BPHYT_RS00385 at 96.623 kb on + strand, within BPHYT_RS00385 at 96.623 kb on + strand, within BPHYT_RS00385 at 96.624 kb on - strand, within BPHYT_RS00385 at 96.654 kb on + strand, within BPHYT_RS00385 at 96.654 kb on + strand, within BPHYT_RS00385 at 96.655 kb on - strand, within BPHYT_RS00385 at 96.658 kb on + strand, within BPHYT_RS00385 at 96.789 kb on + strand, within BPHYT_RS00385 at 96.790 kb on - strand, within BPHYT_RS00385 at 96.790 kb on - strand, within BPHYT_RS00385 at 96.790 kb on - strand, within BPHYT_RS00385 at 96.832 kb on + strand, within BPHYT_RS00385 at 96.993 kb on - strand, within BPHYT_RS00385 at 97.218 kb on + strand, within BPHYT_RS00385
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 94,681 - BPHYT_RS00375 0.11 -1.1 94,874 + BPHYT_RS00375 0.32 +1.2 95,133 + BPHYT_RS00375 0.60 -0.4 95,221 - BPHYT_RS00375 0.70 -1.0 95,397 + BPHYT_RS00375 0.89 +0.1 95,398 - BPHYT_RS00375 0.89 -0.0 95,405 + BPHYT_RS00375 0.90 +1.4 95,406 - BPHYT_RS00375 0.90 -0.0 95,504 - -1.0 95,704 - BPHYT_RS00380 0.12 -1.2 95,965 - BPHYT_RS00380 0.56 -0.4 95,965 - BPHYT_RS00380 0.56 -0.9 96,164 - BPHYT_RS00380 0.89 -1.2 96,382 - BPHYT_RS00385 0.12 -1.2 96,382 - BPHYT_RS00385 0.12 +1.4 96,419 - BPHYT_RS00385 0.15 -1.0 96,468 + BPHYT_RS00385 0.18 -1.6 96,468 + BPHYT_RS00385 0.18 +0.1 96,468 + BPHYT_RS00385 0.18 -3.5 96,469 - BPHYT_RS00385 0.18 -0.7 96,469 - BPHYT_RS00385 0.18 -0.2 96,510 + BPHYT_RS00385 0.21 -0.5 96,565 - BPHYT_RS00385 0.25 +0.1 96,589 + BPHYT_RS00385 0.27 -0.8 96,590 - BPHYT_RS00385 0.27 -0.2 96,623 + BPHYT_RS00385 0.30 -0.3 96,623 + BPHYT_RS00385 0.30 -0.2 96,623 + BPHYT_RS00385 0.30 -0.8 96,623 + BPHYT_RS00385 0.30 -1.7 96,624 - BPHYT_RS00385 0.30 -0.1 96,654 + BPHYT_RS00385 0.32 -1.1 96,654 + BPHYT_RS00385 0.32 -1.0 96,655 - BPHYT_RS00385 0.32 +0.2 96,658 + BPHYT_RS00385 0.32 -0.3 96,789 + BPHYT_RS00385 0.42 +0.5 96,790 - BPHYT_RS00385 0.42 -0.1 96,790 - BPHYT_RS00385 0.42 -1.2 96,790 - BPHYT_RS00385 0.42 +3.1 96,832 + BPHYT_RS00385 0.45 -0.9 96,993 - BPHYT_RS00385 0.57 -0.5 97,218 + BPHYT_RS00385 0.74 -0.1
Or see this region's nucleotide sequence