Experiment: 36hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00360 and BPHYT_RS00365 are separated by 176 nucleotides BPHYT_RS00365 and BPHYT_RS00370 are separated by 26 nucleotides BPHYT_RS00370 and BPHYT_RS00375 overlap by 4 nucleotides
BPHYT_RS00360: BPHYT_RS00360 - protoporphyrinogen oxidase, at 91,249 to 92,604
_RS00360
BPHYT_RS00365: BPHYT_RS00365 - ABC transporter substrate-binding protein, at 92,781 to 93,749
_RS00365
BPHYT_RS00370: BPHYT_RS00370 - ABC transporter ATP-binding protein, at 93,776 to 94,582
_RS00370
BPHYT_RS00375: BPHYT_RS00375 - sugar ABC transporter, at 94,579 to 95,499
_RS00375
Position (kb) 92
93
94 Strain fitness (log2 ratio) -2
-1
0
1
2 at 91.854 kb on + strand, within BPHYT_RS00360 at 91.874 kb on + strand, within BPHYT_RS00360 at 91.874 kb on + strand, within BPHYT_RS00360 at 91.875 kb on - strand, within BPHYT_RS00360 at 92.065 kb on - strand, within BPHYT_RS00360 at 92.066 kb on + strand, within BPHYT_RS00360 at 92.067 kb on - strand, within BPHYT_RS00360 at 92.119 kb on + strand, within BPHYT_RS00360 at 92.120 kb on - strand, within BPHYT_RS00360 at 92.242 kb on + strand, within BPHYT_RS00360 at 92.243 kb on - strand, within BPHYT_RS00360 at 92.328 kb on - strand, within BPHYT_RS00360 at 92.579 kb on + strand at 92.707 kb on + strand at 93.105 kb on - strand, within BPHYT_RS00365 at 93.424 kb on - strand, within BPHYT_RS00365 at 93.493 kb on + strand, within BPHYT_RS00365 at 93.549 kb on + strand, within BPHYT_RS00365 at 93.660 kb on + strand at 93.661 kb on - strand at 93.910 kb on - strand, within BPHYT_RS00370 at 93.968 kb on - strand, within BPHYT_RS00370 at 93.968 kb on - strand, within BPHYT_RS00370 at 94.009 kb on + strand, within BPHYT_RS00370 at 94.041 kb on - strand, within BPHYT_RS00370 at 94.442 kb on + strand, within BPHYT_RS00370 at 94.681 kb on - strand, within BPHYT_RS00375
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 1 remove 91,854 + BPHYT_RS00360 0.45 -0.2 91,874 + BPHYT_RS00360 0.46 -0.3 91,874 + BPHYT_RS00360 0.46 -0.2 91,875 - BPHYT_RS00360 0.46 +1.8 92,065 - BPHYT_RS00360 0.60 -0.7 92,066 + BPHYT_RS00360 0.60 +0.2 92,067 - BPHYT_RS00360 0.60 +0.5 92,119 + BPHYT_RS00360 0.64 +0.1 92,120 - BPHYT_RS00360 0.64 +0.1 92,242 + BPHYT_RS00360 0.73 -1.2 92,243 - BPHYT_RS00360 0.73 +1.4 92,328 - BPHYT_RS00360 0.80 -1.1 92,579 + -0.9 92,707 + -2.0 93,105 - BPHYT_RS00365 0.33 -0.0 93,424 - BPHYT_RS00365 0.66 -0.4 93,493 + BPHYT_RS00365 0.73 -2.4 93,549 + BPHYT_RS00365 0.79 -0.2 93,660 + +0.8 93,661 - -1.5 93,910 - BPHYT_RS00370 0.17 +1.9 93,968 - BPHYT_RS00370 0.24 +0.1 93,968 - BPHYT_RS00370 0.24 +1.2 94,009 + BPHYT_RS00370 0.29 -0.2 94,041 - BPHYT_RS00370 0.33 +1.3 94,442 + BPHYT_RS00370 0.83 -0.4 94,681 - BPHYT_RS00375 0.11 -1.1
Or see this region's nucleotide sequence