Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36350 and BPHYT_RS36355 are separated by 246 nucleotides BPHYT_RS36355 and BPHYT_RS36360 are separated by 13 nucleotides BPHYT_RS36360 and BPHYT_RS36365 are separated by 153 nucleotides BPHYT_RS36365 and BPHYT_RS36370 are separated by 230 nucleotides
BPHYT_RS36350: BPHYT_RS36350 - hypothetical protein, at 83,145 to 84,278
_RS36350
BPHYT_RS36355: BPHYT_RS36355 - hypothetical protein, at 84,525 to 84,875
_RS36355
BPHYT_RS36360: BPHYT_RS36360 - hypothetical protein, at 84,889 to 85,194
_RS36360
BPHYT_RS36365: BPHYT_RS36365 - hypothetical protein, at 85,348 to 85,650
_RS36365
BPHYT_RS36370: BPHYT_RS36370 - integrase, at 85,881 to 87,089
_RS36370
Position (kb) 84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 83.914 kb on - strand, within BPHYT_RS36350 at 84.004 kb on - strand, within BPHYT_RS36350 at 84.423 kb on - strand at 84.801 kb on + strand, within BPHYT_RS36355 at 84.803 kb on - strand, within BPHYT_RS36355 at 84.980 kb on - strand, within BPHYT_RS36360 at 84.997 kb on + strand, within BPHYT_RS36360 at 85.099 kb on + strand, within BPHYT_RS36360 at 85.619 kb on + strand, within BPHYT_RS36365 at 85.675 kb on + strand at 85.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 83,914 - BPHYT_RS36350 0.68 +0.3 84,004 - BPHYT_RS36350 0.76 -0.8 84,423 - +0.8 84,801 + BPHYT_RS36355 0.79 -0.9 84,803 - BPHYT_RS36355 0.79 -0.1 84,980 - BPHYT_RS36360 0.30 +0.1 84,997 + BPHYT_RS36360 0.35 -1.1 85,099 + BPHYT_RS36360 0.69 +0.6 85,619 + BPHYT_RS36365 0.89 -3.0 85,675 + +1.6 85,990 - +0.6
Or see this region's nucleotide sequence