Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30880 and BPHYT_RS30885 are separated by 156 nucleotides BPHYT_RS30885 and BPHYT_RS30890 are separated by 52 nucleotides BPHYT_RS30890 and BPHYT_RS30895 are separated by 128 nucleotides BPHYT_RS30895 and BPHYT_RS30900 are separated by 417 nucleotides
BPHYT_RS30880: BPHYT_RS30880 - peptidase S13, at 2,482,140 to 2,483,774
_RS30880
BPHYT_RS30885: BPHYT_RS30885 - hypothetical protein, at 2,483,931 to 2,484,239
_RS30885
BPHYT_RS30890: BPHYT_RS30890 - hypothetical protein, at 2,484,292 to 2,485,062
_RS30890
BPHYT_RS30895: BPHYT_RS30895 - hypothetical protein, at 2,485,191 to 2,485,400
_RS30895
BPHYT_RS30900: BPHYT_RS30900 - transcriptional regulator, at 2,485,818 to 2,486,450
_RS30900
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-1
0
1 at 2483.330 kb on + strand, within BPHYT_RS30880 at 2483.940 kb on + strand at 2484.040 kb on - strand, within BPHYT_RS30885 at 2484.040 kb on - strand, within BPHYT_RS30885 at 2484.140 kb on + strand, within BPHYT_RS30885 at 2484.723 kb on + strand, within BPHYT_RS30890 at 2485.600 kb on - strand at 2485.984 kb on - strand, within BPHYT_RS30900 at 2485.987 kb on - strand, within BPHYT_RS30900
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 2,483,330 + BPHYT_RS30880 0.73 -1.2 2,483,940 + -0.4 2,484,040 - BPHYT_RS30885 0.35 +1.6 2,484,040 - BPHYT_RS30885 0.35 +1.5 2,484,140 + BPHYT_RS30885 0.68 -0.5 2,484,723 + BPHYT_RS30890 0.56 -1.3 2,485,600 - +1.3 2,485,984 - BPHYT_RS30900 0.26 -0.0 2,485,987 - BPHYT_RS30900 0.27 -0.2
Or see this region's nucleotide sequence