Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27575 and BPHYT_RS27580 overlap by 4 nucleotides BPHYT_RS27580 and BPHYT_RS27585 overlap by 14 nucleotides BPHYT_RS27585 and BPHYT_RS27590 are separated by 9 nucleotides BPHYT_RS27590 and BPHYT_RS27595 overlap by 4 nucleotides
BPHYT_RS27575: BPHYT_RS27575 - ATP synthase F1 subunit gamma, at 1,759,233 to 1,760,081
_RS27575
BPHYT_RS27580: BPHYT_RS27580 - F0F1 ATP synthase subunit alpha, at 1,760,078 to 1,761,646
_RS27580
BPHYT_RS27585: BPHYT_RS27585 - ATP synthase F0 subunit B, at 1,761,633 to 1,762,382
_RS27585
BPHYT_RS27590: BPHYT_RS27590 - F0F1 ATP synthase subunit C, at 1,762,392 to 1,762,640
_RS27590
BPHYT_RS27595: BPHYT_RS27595 - F0F1 ATP synthase subunit A, at 1,762,637 to 1,763,335
_RS27595
Position (kb) 1760
1761
1762 Strain fitness (log2 ratio) -1
0
1
2 at 1759.429 kb on + strand, within BPHYT_RS27575 at 1759.429 kb on + strand, within BPHYT_RS27575 at 1759.435 kb on + strand, within BPHYT_RS27575 at 1759.560 kb on - strand, within BPHYT_RS27575 at 1759.788 kb on + strand, within BPHYT_RS27575 at 1760.481 kb on + strand, within BPHYT_RS27580 at 1760.895 kb on + strand, within BPHYT_RS27580 at 1760.995 kb on - strand, within BPHYT_RS27580 at 1762.444 kb on + strand, within BPHYT_RS27590
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 1,759,429 + BPHYT_RS27575 0.23 -0.3 1,759,429 + BPHYT_RS27575 0.23 +1.5 1,759,435 + BPHYT_RS27575 0.24 +0.7 1,759,560 - BPHYT_RS27575 0.39 -0.3 1,759,788 + BPHYT_RS27575 0.65 +2.0 1,760,481 + BPHYT_RS27580 0.26 -0.2 1,760,895 + BPHYT_RS27580 0.52 -0.1 1,760,995 - BPHYT_RS27580 0.58 +0.8 1,762,444 + BPHYT_RS27590 0.21 +0.8
Or see this region's nucleotide sequence