Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26960 and BPHYT_RS26965 are separated by 73 nucleotides BPHYT_RS26965 and BPHYT_RS26970 overlap by 14 nucleotides BPHYT_RS26970 and BPHYT_RS26975 overlap by 4 nucleotides BPHYT_RS26975 and BPHYT_RS26980 are separated by 237 nucleotides
BPHYT_RS26960: BPHYT_RS26960 - LysR family transcriptional regulator, at 1,626,585 to 1,627,469
_RS26960
BPHYT_RS26965: BPHYT_RS26965 - L-lactate dehydrogenase, LldG subunit (from data) , at 1,627,543 to 1,628,130
_RS26965
BPHYT_RS26970: BPHYT_RS26970 - L-lactate dehydrogenase, LldF subunit (from data) , at 1,628,117 to 1,629,511
_RS26970
BPHYT_RS26975: BPHYT_RS26975 - L-lactate dehydrogenase, LldE subunit (from data) , at 1,629,508 to 1,630,251
_RS26975
BPHYT_RS26980: BPHYT_RS26980 - HipA protein, at 1,630,489 to 1,631,808
_RS26980
Position (kb)
1628
1629
1630 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1627.279 kb on + strand, within BPHYT_RS26960 at 1627.769 kb on + strand, within BPHYT_RS26965 at 1628.212 kb on + strand at 1628.213 kb on - strand at 1628.225 kb on - strand at 1628.286 kb on + strand, within BPHYT_RS26970 at 1628.385 kb on + strand, within BPHYT_RS26970 at 1628.386 kb on - strand, within BPHYT_RS26970 at 1628.467 kb on + strand, within BPHYT_RS26970 at 1628.569 kb on + strand, within BPHYT_RS26970 at 1628.570 kb on - strand at 1628.706 kb on + strand, within BPHYT_RS26970 at 1628.865 kb on - strand, within BPHYT_RS26970 at 1629.315 kb on + strand, within BPHYT_RS26970 at 1629.624 kb on - strand, within BPHYT_RS26975 at 1629.858 kb on + strand, within BPHYT_RS26975 at 1630.292 kb on + strand at 1630.293 kb on - strand at 1630.404 kb on + strand at 1630.406 kb on + strand at 1630.500 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 1,627,279 + BPHYT_RS26960 0.78 -0.5 1,627,769 + BPHYT_RS26965 0.38 +0.5 1,628,212 + -0.4 1,628,213 - -0.1 1,628,225 - -1.1 1,628,286 + BPHYT_RS26970 0.12 -1.1 1,628,385 + BPHYT_RS26970 0.19 -0.0 1,628,386 - BPHYT_RS26970 0.19 +0.8 1,628,467 + BPHYT_RS26970 0.25 +3.4 1,628,569 + BPHYT_RS26970 0.32 +1.2 1,628,570 - +2.7 1,628,706 + BPHYT_RS26970 0.42 +0.9 1,628,865 - BPHYT_RS26970 0.54 +0.8 1,629,315 + BPHYT_RS26970 0.86 +1.7 1,629,624 - BPHYT_RS26975 0.16 +1.5 1,629,858 + BPHYT_RS26975 0.47 +1.2 1,630,292 + -1.1 1,630,293 - +0.8 1,630,404 + -0.5 1,630,406 + -0.2 1,630,500 + +1.0
Or see this region's nucleotide sequence