Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10940 and BPHYT_RS10945 are separated by 40 nucleotides BPHYT_RS10945 and BPHYT_RS10950 are separated by 123 nucleotides
BPHYT_RS10940: BPHYT_RS10940 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, at 2,489,968 to 2,490,804
_RS10940
BPHYT_RS10945: BPHYT_RS10945 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,490,845 to 2,491,615
_RS10945
BPHYT_RS10950: BPHYT_RS10950 - transcriptional regulator, at 2,491,739 to 2,492,611
_RS10950
Position (kb)
2490
2491
2492 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.091 kb on - strand, within BPHYT_RS10940 at 2490.581 kb on - strand, within BPHYT_RS10940 at 2490.586 kb on + strand, within BPHYT_RS10940 at 2490.725 kb on - strand at 2490.735 kb on - strand at 2491.325 kb on + strand, within BPHYT_RS10945 at 2491.422 kb on - strand, within BPHYT_RS10945 at 2491.579 kb on + strand at 2491.580 kb on - strand at 2491.580 kb on - strand at 2491.683 kb on + strand at 2492.150 kb on + strand, within BPHYT_RS10950 at 2492.151 kb on - strand, within BPHYT_RS10950 at 2492.221 kb on + strand, within BPHYT_RS10950 at 2492.222 kb on - strand, within BPHYT_RS10950
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 2,490,082 + BPHYT_RS10940 0.14 +0.0 2,490,082 + BPHYT_RS10940 0.14 +1.6 2,490,083 - BPHYT_RS10940 0.14 +0.6 2,490,083 - BPHYT_RS10940 0.14 -0.1 2,490,083 - BPHYT_RS10940 0.14 +0.0 2,490,091 - BPHYT_RS10940 0.15 +0.7 2,490,581 - BPHYT_RS10940 0.73 -0.1 2,490,586 + BPHYT_RS10940 0.74 +1.0 2,490,725 - +3.2 2,490,735 - -1.2 2,491,325 + BPHYT_RS10945 0.62 -1.3 2,491,422 - BPHYT_RS10945 0.75 -2.4 2,491,579 + +0.7 2,491,580 - -1.4 2,491,580 - +0.0 2,491,683 + -1.1 2,492,150 + BPHYT_RS10950 0.47 +0.8 2,492,151 - BPHYT_RS10950 0.47 +2.4 2,492,221 + BPHYT_RS10950 0.55 +0.7 2,492,222 - BPHYT_RS10950 0.55 +0.4
Or see this region's nucleotide sequence